This study is designed to evaluate whether conjugated linoleic acid-coupled Pluronic F127 (Plu-CLA) enhances anticancer efficacy in MCF-7 breast cancer cells when compared to conjugated linoleic acid (CLA) itself. CLA was simply coupled to Pluronic F127 through ester linkage between carboxyl group of CLA and hydroxyl one of Pluronic at melting state without solvent or catalyst. Plu-CLA significantly enhanced apoptosis with increasing concentration compared with CLA itself. Moreover, it was found that p53, p21, and Bax were up-regulated, whereas Bcl-2 and procaspase 9 were down-regulated with increasing concentration of Plu-CLA. These results were attributed to the sensitization activity of Pluronic F127.
Bibliographical noteFunding Information:
This work was supported by the Agricultural R&D Promotion Center (ARPC) (2006-0053). We acknowledge National Instrumentation Center for Environmental Management (NICEM) for providing opportunity for measurement of NMR.
- Conjugated linoleic acid (CLA)
- Pluronic F127
- Polymer conjugation
ASJC Scopus subject areas
- Cancer Research