Enhanced biomass and lipid production of Neochloris oleoabundans under high light conditions by anisotropic nature of light-splitting CaCO3 crystal

Min Eui Hong; Byung Sun Yu; Anil Kumar Patel; Hong Il Choi; Sojin Song; Young Joon Sung; Won Seok Chang; Sang Jun Sim

doi:10.1016/j.biortech.2019.121483

Enhanced biomass and lipid production of Neochloris oleoabundans under high light conditions by anisotropic nature of light-splitting CaCO₃ crystal

Min Eui Hong, Byung Sun Yu, Anil Kumar Patel, Hong Il Choi, Sojin Song, Young Joon Sung, Won Seok Chang, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The aim of this work was to study the anisotropic effect of crystalline CaCO₃ nanoparticles (CN)-driven multiple refraction/scattering from the CN-coated agglomerated cells on the rate of photosynthesis and the product yield under high light conditions in the freshwater microalgae Neochloris oleoabundans. The CN-coating via biomineralization significantly improved the biomass and lipid production of N. oleoabundans during second stage of autotrophic induction by sustaining relatively high rate of photosynthesis at high irradiance using the multiple-splitting effect of the anisotropic polymorphism. The CN were successfully produced, adsorbed and grown on the external cells under conditions of mild alkalinity (pH 7.5–8.0), mild CaCl₂ concentration (0.05 M) and under nitrogen starvation with strong light (400 µE m⁻² s⁻¹). Consequently, lipid content and productivity of N. oleoabundans cells cultured with 0.05 M CaCl₂ increased by 18.4% and 31.5%, respectively, compared to the cells cultured with 0.05 M CaCl₂ and acetazolamide to inhibit calcification.

Original language	English
Article number	121483
Journal	Bioresource technology
Volume	287
DOIs	https://doi.org/10.1016/j.biortech.2019.121483
Publication status	Published - 2019 Sept

Bibliographical note

Funding Information:
The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC - 2014M1A8A1049278 ). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government ( Ministry of Trade, Industry and Energy ) (Grant Number: 20172010202050 ). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC- 1003542018S075 ).

Funding Information:
The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC-2014M1A8A1049278). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government (Ministry of Trade, Industry and Energy) (Grant Number: 20172010202050). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC-1003542018S075).

Keywords

Anisotropic CaCO crystal
CaCO biomineralization
Mild calcification method
Multiple light-splitting effect
Neochloris oleoabundans

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2019.121483

Cite this

@article{4a86afd314c24b60ad99710264995557,

title = "Enhanced biomass and lipid production of Neochloris oleoabundans under high light conditions by anisotropic nature of light-splitting CaCO3 crystal",

abstract = "The aim of this work was to study the anisotropic effect of crystalline CaCO3 nanoparticles (CN)-driven multiple refraction/scattering from the CN-coated agglomerated cells on the rate of photosynthesis and the product yield under high light conditions in the freshwater microalgae Neochloris oleoabundans. The CN-coating via biomineralization significantly improved the biomass and lipid production of N. oleoabundans during second stage of autotrophic induction by sustaining relatively high rate of photosynthesis at high irradiance using the multiple-splitting effect of the anisotropic polymorphism. The CN were successfully produced, adsorbed and grown on the external cells under conditions of mild alkalinity (pH 7.5–8.0), mild CaCl2 concentration (0.05 M) and under nitrogen starvation with strong light (400 µE m−2 s−1). Consequently, lipid content and productivity of N. oleoabundans cells cultured with 0.05 M CaCl2 increased by 18.4% and 31.5%, respectively, compared to the cells cultured with 0.05 M CaCl2 and acetazolamide to inhibit calcification.",

keywords = "Anisotropic CaCO crystal, CaCO biomineralization, Mild calcification method, Multiple light-splitting effect, Neochloris oleoabundans",

author = "Hong, {Min Eui} and Yu, {Byung Sun} and Patel, {Anil Kumar} and Choi, {Hong Il} and Sojin Song and Sung, {Young Joon} and Chang, {Won Seok} and Sim, {Sang Jun}",

note = "Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC - 2014M1A8A1049278 ). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government ( Ministry of Trade, Industry and Energy ) (Grant Number: 20172010202050 ). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC- 1003542018S075 ). Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC-2014M1A8A1049278). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government (Ministry of Trade, Industry and Energy) (Grant Number: 20172010202050). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC-1003542018S075).",

year = "2019",

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doi = "10.1016/j.biortech.2019.121483",

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T1 - Enhanced biomass and lipid production of Neochloris oleoabundans under high light conditions by anisotropic nature of light-splitting CaCO3 crystal

AU - Hong, Min Eui

AU - Yu, Byung Sun

AU - Patel, Anil Kumar

AU - Choi, Hong Il

AU - Song, Sojin

AU - Sung, Young Joon

AU - Chang, Won Seok

AU - Sim, Sang Jun

N1 - Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC - 2014M1A8A1049278 ). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government ( Ministry of Trade, Industry and Energy ) (Grant Number: 20172010202050 ). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC- 1003542018S075 ). Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC-2014M1A8A1049278). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government (Ministry of Trade, Industry and Energy) (Grant Number: 20172010202050). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC-1003542018S075).

PY - 2019/9

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N2 - The aim of this work was to study the anisotropic effect of crystalline CaCO3 nanoparticles (CN)-driven multiple refraction/scattering from the CN-coated agglomerated cells on the rate of photosynthesis and the product yield under high light conditions in the freshwater microalgae Neochloris oleoabundans. The CN-coating via biomineralization significantly improved the biomass and lipid production of N. oleoabundans during second stage of autotrophic induction by sustaining relatively high rate of photosynthesis at high irradiance using the multiple-splitting effect of the anisotropic polymorphism. The CN were successfully produced, adsorbed and grown on the external cells under conditions of mild alkalinity (pH 7.5–8.0), mild CaCl2 concentration (0.05 M) and under nitrogen starvation with strong light (400 µE m−2 s−1). Consequently, lipid content and productivity of N. oleoabundans cells cultured with 0.05 M CaCl2 increased by 18.4% and 31.5%, respectively, compared to the cells cultured with 0.05 M CaCl2 and acetazolamide to inhibit calcification.

AB - The aim of this work was to study the anisotropic effect of crystalline CaCO3 nanoparticles (CN)-driven multiple refraction/scattering from the CN-coated agglomerated cells on the rate of photosynthesis and the product yield under high light conditions in the freshwater microalgae Neochloris oleoabundans. The CN-coating via biomineralization significantly improved the biomass and lipid production of N. oleoabundans during second stage of autotrophic induction by sustaining relatively high rate of photosynthesis at high irradiance using the multiple-splitting effect of the anisotropic polymorphism. The CN were successfully produced, adsorbed and grown on the external cells under conditions of mild alkalinity (pH 7.5–8.0), mild CaCl2 concentration (0.05 M) and under nitrogen starvation with strong light (400 µE m−2 s−1). Consequently, lipid content and productivity of N. oleoabundans cells cultured with 0.05 M CaCl2 increased by 18.4% and 31.5%, respectively, compared to the cells cultured with 0.05 M CaCl2 and acetazolamide to inhibit calcification.

KW - Anisotropic CaCO crystal

KW - CaCO biomineralization

KW - Mild calcification method

KW - Multiple light-splitting effect

KW - Neochloris oleoabundans

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