Enhanced biomass and lipid production of Neochloris oleoabundans under high light conditions by anisotropic nature of light-splitting CaCO3 crystal

Min Eui Hong, Byung Sun Yu, Anil Kumar Patel, Hong Il Choi, Sojin Song, Young Joon Sung, Won Seok Chang, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

The aim of this work was to study the anisotropic effect of crystalline CaCO3 nanoparticles (CN)-driven multiple refraction/scattering from the CN-coated agglomerated cells on the rate of photosynthesis and the product yield under high light conditions in the freshwater microalgae Neochloris oleoabundans. The CN-coating via biomineralization significantly improved the biomass and lipid production of N. oleoabundans during second stage of autotrophic induction by sustaining relatively high rate of photosynthesis at high irradiance using the multiple-splitting effect of the anisotropic polymorphism. The CN were successfully produced, adsorbed and grown on the external cells under conditions of mild alkalinity (pH 7.5–8.0), mild CaCl2 concentration (0.05 M) and under nitrogen starvation with strong light (400 µE m−2 s−1). Consequently, lipid content and productivity of N. oleoabundans cells cultured with 0.05 M CaCl2 increased by 18.4% and 31.5%, respectively, compared to the cells cultured with 0.05 M CaCl2 and acetazolamide to inhibit calcification.

Original languageEnglish
Article number121483
JournalBioresource technology
Volume287
DOIs
Publication statusPublished - 2019 Sept

Bibliographical note

Funding Information:
The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC - 2014M1A8A1049278 ). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government ( Ministry of Trade, Industry and Energy ) (Grant Number: 20172010202050 ). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC- 1003542018S075 ).

Funding Information:
The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC-2014M1A8A1049278). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2019R1A2C3009821) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government (Ministry of Trade, Industry and Energy) (Grant Number: 20172010202050). The authors specially thank to Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (grant number: KDHC-1003542018S075).

Keywords

  • Anisotropic CaCO crystal
  • CaCO biomineralization
  • Mild calcification method
  • Multiple light-splitting effect
  • Neochloris oleoabundans

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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