Enhanced biomass production through a repeated sequential auto-and heterotrophic culture mode in Chlorella protothecoides

Jaemin Joun, Min Eui Hong, Ranjna Sirohi, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


A repeated sequential auto-and heterotrophic (RSAH) culture mode was designed to enhance biomass of Chlorella protothecoides. Based on the result that the photosynthesis system may receive damage if the light period is more than 16 h, autotrophy was applied in the 16 h of the light cycle and mixotrophy using acetic acid and glucose in the 8 h of dark cycle. In the dark cycle, an organic carbon source was added according to the Monod equation to maintain activation of the TCA cycle and organic carbon source-to-cell conversion. When acetic acid and glucose were used as organic carbon sources, this culture method was found to be 32.3% and 12.6% higher in biomass, 2.59 and 2.67 times higher in the organic carbon source-to-cell conversion factor, and 2.17 and 2.32 times higher in ATP/ADP ratio, respectively, compared to mixotrophy. Through this new culture method, economic feasibility and carbon reduction capabilities in large-scale cultures can be achieved.

Original languageEnglish
Article number125532
JournalBioresource technology
Publication statusPublished - 2021 Oct

Bibliographical note

Funding Information:
This study was supported by the “Carbon to X” project (no. 2020M3H7A1098295), which is funded by the National Research Foundation (NRF) and the Ministry of Science and ICT, Republic of Korea , the Korea Institute of Energy Technology Evaluation and Planning (KETEP) (no. 20172010202050 ), and the Korea University Grant .

Publisher Copyright:
© 2021 Elsevier Ltd


  • Acetic acid
  • Chlorella
  • Glucose
  • Mixotrophic
  • Repeated sequential auto-and heterotrophic culture mode

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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