Enhanced CO2 fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

Seung Kyou You; Young Jin Ko; Sang Kyu Shin; Dong hyeuk Hwang; Dae Hee Kang; Hyeon Min Park; Sung Ok Han

doi:10.1016/j.biortech.2020.124072

Enhanced CO₂ fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

Seung Kyou You, Young Jin Ko, Sang Kyu Shin, Dong hyeuk Hwang, Dae Hee Kang, Hyeon Min Park, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Photosynthesis of C. vulgaris shows slow growth and low lipid production due to the low solubility of CO₂, and it is thus necessary to increase the dissolved inorganic carbon source to solve this problem. In this study, carbonic anhydrase (CA) was fused with dockerin to form a CA complex by cohesion-dockerin interaction. The CA complex was displayed on the surface of C. vulgaris by a cellulose binding module. The CA complex increased activity and stability compared to those of a single enzyme. Additionally, C. vulgaris showed an average of 1.6-fold rapid growth during log phase through the influence of the CA complex. The bicarbonate produced by the CA complex increased the lipid production about 1.7-fold (23.3%), compared to 13.6% for the control group. The present results suggest that the CA complex successfully enhances the CO₂ fixation, which should be an essential study for 4th generation biofuels.

Original language	English
Article number	124072
Journal	Bioresource technology
Volume	318
DOIs	https://doi.org/10.1016/j.biortech.2020.124072
Publication status	Published - 2020 Dec

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. 2018R1A2B2003704) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus.

Funding Information:
This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) (no. 2018R1A2B2003704 ) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus.

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

CO fixation
Carbonic anhydrase complex
Chlorella vulgaris
Lipid

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2020.124072

Cite this

@article{6edda7cbb142449bb5baf81d1f4c329e,

title = "Enhanced CO2 fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex",

abstract = "Photosynthesis of C. vulgaris shows slow growth and low lipid production due to the low solubility of CO2, and it is thus necessary to increase the dissolved inorganic carbon source to solve this problem. In this study, carbonic anhydrase (CA) was fused with dockerin to form a CA complex by cohesion-dockerin interaction. The CA complex was displayed on the surface of C. vulgaris by a cellulose binding module. The CA complex increased activity and stability compared to those of a single enzyme. Additionally, C. vulgaris showed an average of 1.6-fold rapid growth during log phase through the influence of the CA complex. The bicarbonate produced by the CA complex increased the lipid production about 1.7-fold (23.3%), compared to 13.6% for the control group. The present results suggest that the CA complex successfully enhances the CO2 fixation, which should be an essential study for 4th generation biofuels.",

keywords = "CO fixation, Carbonic anhydrase complex, Chlorella vulgaris, Lipid",

author = "You, {Seung Kyou} and Ko, {Young Jin} and Shin, {Sang Kyu} and Hwang, {Dong hyeuk} and Kang, {Dae Hee} and Park, {Hyeon Min} and Han, {Sung Ok}",

note = "Funding Information: This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. 2018R1A2B2003704) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus. Funding Information: This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) (no. 2018R1A2B2003704 ) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = dec,

doi = "10.1016/j.biortech.2020.124072",

language = "English",

volume = "318",

journal = "Bioresource technology",

issn = "0960-8524",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Enhanced CO2 fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

AU - You, Seung Kyou

AU - Ko, Young Jin

AU - Shin, Sang Kyu

AU - Hwang, Dong hyeuk

AU - Kang, Dae Hee

AU - Park, Hyeon Min

AU - Han, Sung Ok

N1 - Funding Information: This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. 2018R1A2B2003704) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus. Funding Information: This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) (no. 2018R1A2B2003704 ) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus. Publisher Copyright: © 2020 Elsevier Ltd

PY - 2020/12

Y1 - 2020/12

N2 - Photosynthesis of C. vulgaris shows slow growth and low lipid production due to the low solubility of CO2, and it is thus necessary to increase the dissolved inorganic carbon source to solve this problem. In this study, carbonic anhydrase (CA) was fused with dockerin to form a CA complex by cohesion-dockerin interaction. The CA complex was displayed on the surface of C. vulgaris by a cellulose binding module. The CA complex increased activity and stability compared to those of a single enzyme. Additionally, C. vulgaris showed an average of 1.6-fold rapid growth during log phase through the influence of the CA complex. The bicarbonate produced by the CA complex increased the lipid production about 1.7-fold (23.3%), compared to 13.6% for the control group. The present results suggest that the CA complex successfully enhances the CO2 fixation, which should be an essential study for 4th generation biofuels.

AB - Photosynthesis of C. vulgaris shows slow growth and low lipid production due to the low solubility of CO2, and it is thus necessary to increase the dissolved inorganic carbon source to solve this problem. In this study, carbonic anhydrase (CA) was fused with dockerin to form a CA complex by cohesion-dockerin interaction. The CA complex was displayed on the surface of C. vulgaris by a cellulose binding module. The CA complex increased activity and stability compared to those of a single enzyme. Additionally, C. vulgaris showed an average of 1.6-fold rapid growth during log phase through the influence of the CA complex. The bicarbonate produced by the CA complex increased the lipid production about 1.7-fold (23.3%), compared to 13.6% for the control group. The present results suggest that the CA complex successfully enhances the CO2 fixation, which should be an essential study for 4th generation biofuels.

KW - CO fixation

KW - Carbonic anhydrase complex

KW - Chlorella vulgaris

KW - Lipid

UR - http://www.scopus.com/inward/record.url?scp=85090368033&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2020.124072

DO - 10.1016/j.biortech.2020.124072

M3 - Article

C2 - 32911368

AN - SCOPUS:85090368033

SN - 0960-8524

VL - 318

JO - Bioresource technology

JF - Bioresource technology

M1 - 124072

ER -