Abstract
Photosynthesis of C. vulgaris shows slow growth and low lipid production due to the low solubility of CO2, and it is thus necessary to increase the dissolved inorganic carbon source to solve this problem. In this study, carbonic anhydrase (CA) was fused with dockerin to form a CA complex by cohesion-dockerin interaction. The CA complex was displayed on the surface of C. vulgaris by a cellulose binding module. The CA complex increased activity and stability compared to those of a single enzyme. Additionally, C. vulgaris showed an average of 1.6-fold rapid growth during log phase through the influence of the CA complex. The bicarbonate produced by the CA complex increased the lipid production about 1.7-fold (23.3%), compared to 13.6% for the control group. The present results suggest that the CA complex successfully enhances the CO2 fixation, which should be an essential study for 4th generation biofuels.
Original language | English |
---|---|
Article number | 124072 |
Journal | Bioresource technology |
Volume | 318 |
DOIs | |
Publication status | Published - 2020 Dec |
Bibliographical note
Funding Information:This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. 2018R1A2B2003704) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus.
Funding Information:
This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) (no. 2018R1A2B2003704 ) and Korea University Grant. Also, we thank Professor Hyung Joon Cha of the Department of Chemical Engineering at POSTECH for providing the carbonic anhydrase gene source of Hydrogenovibrio marinus.
Publisher Copyright:
© 2020 Elsevier Ltd
Keywords
- CO fixation
- Carbonic anhydrase complex
- Chlorella vulgaris
- Lipid
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal