Enhanced effects of osteoclastogenesis inhibition by curcumin-delivering heparin nanoparticles

Young Pil Yun; Sung Eun Kim; Jae Yong Lee; Hak Jun Kim; Sung Wook Choi; Hae Ryong Song; Kyeongsoon Park

doi:10.1007/s13233-014-2082-1

Enhanced effects of osteoclastogenesis inhibition by curcumin-delivering heparin nanoparticles

Young Pil Yun, Sung Eun Kim, Jae Yong Lee, Hak Jun Kim, Sung Wook Choi, Hae Ryong Song, Kyeongsoon Park

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Curcumin has various pharmacological activities such as anti-inflammatory, antioxidant, antimicrobial and antitumor activity. However, its use has been limited due to its poor solubility in water and minimal systemic bioavailability. Thus, we developed curcumin-delivering heparin-based nanoparticles and evaluated the inhibition effects of osteoclastogenesis by curcumin-delivering heparin nanoparticles (Cur-HD NPs). Cur-HD NPs were dispersed well in aqueous solution by forming self-assembled nanoparticles and showed sustained release of curcumin. In vitro studies, HD NPs facilitated intracellular delivery of curcumin into macrophages and osteoclasts, and thus, Cur-HD NPs effectively inhibited osteoclastogenesis in a dose-dependent manner by suppressing tartrate-resistant acid phosphatase (TRAP) activity and TRAP-positive multinucleated cells as well as by reducing the expression of osteoclast marker genes (i.e., TRAP and nuclear factor of activated T cells cytoplasmic 1 (NFATc1)). Furthermore, Cur-HD NPs markedly stimulated apoptosis of osteoclasts. Therefore, we hope that Cur-HD NPs will be useful nanodrugs for the treatment of bone-related diseases.

Original language	English
Pages (from-to)	647-656
Number of pages	10
Journal	Macromolecular Research
Volume	22
Issue number	6
DOIs	https://doi.org/10.1007/s13233-014-2082-1
Publication status	Published - 2014 Jun

Keywords

RANKL
curcumin
heparin nanoparticles
osteoclast
osteoclastogenesis

ASJC Scopus subject areas

Chemical Engineering(all)
Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1007/s13233-014-2082-1

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title = "Enhanced effects of osteoclastogenesis inhibition by curcumin-delivering heparin nanoparticles",

abstract = "Curcumin has various pharmacological activities such as anti-inflammatory, antioxidant, antimicrobial and antitumor activity. However, its use has been limited due to its poor solubility in water and minimal systemic bioavailability. Thus, we developed curcumin-delivering heparin-based nanoparticles and evaluated the inhibition effects of osteoclastogenesis by curcumin-delivering heparin nanoparticles (Cur-HD NPs). Cur-HD NPs were dispersed well in aqueous solution by forming self-assembled nanoparticles and showed sustained release of curcumin. In vitro studies, HD NPs facilitated intracellular delivery of curcumin into macrophages and osteoclasts, and thus, Cur-HD NPs effectively inhibited osteoclastogenesis in a dose-dependent manner by suppressing tartrate-resistant acid phosphatase (TRAP) activity and TRAP-positive multinucleated cells as well as by reducing the expression of osteoclast marker genes (i.e., TRAP and nuclear factor of activated T cells cytoplasmic 1 (NFATc1)). Furthermore, Cur-HD NPs markedly stimulated apoptosis of osteoclasts. Therefore, we hope that Cur-HD NPs will be useful nanodrugs for the treatment of bone-related diseases.",

keywords = "RANKL, curcumin, heparin nanoparticles, osteoclast, osteoclastogenesis",

author = "Yun, {Young Pil} and Kim, {Sung Eun} and Lee, {Jae Yong} and Kim, {Hak Jun} and Choi, {Sung Wook} and Song, {Hae Ryong} and Kyeongsoon Park",

year = "2014",

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doi = "10.1007/s13233-014-2082-1",

language = "English",

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AU - Yun, Young Pil

AU - Kim, Sung Eun

AU - Lee, Jae Yong

AU - Kim, Hak Jun

AU - Choi, Sung Wook

AU - Song, Hae Ryong

AU - Park, Kyeongsoon

PY - 2014/6

Y1 - 2014/6

N2 - Curcumin has various pharmacological activities such as anti-inflammatory, antioxidant, antimicrobial and antitumor activity. However, its use has been limited due to its poor solubility in water and minimal systemic bioavailability. Thus, we developed curcumin-delivering heparin-based nanoparticles and evaluated the inhibition effects of osteoclastogenesis by curcumin-delivering heparin nanoparticles (Cur-HD NPs). Cur-HD NPs were dispersed well in aqueous solution by forming self-assembled nanoparticles and showed sustained release of curcumin. In vitro studies, HD NPs facilitated intracellular delivery of curcumin into macrophages and osteoclasts, and thus, Cur-HD NPs effectively inhibited osteoclastogenesis in a dose-dependent manner by suppressing tartrate-resistant acid phosphatase (TRAP) activity and TRAP-positive multinucleated cells as well as by reducing the expression of osteoclast marker genes (i.e., TRAP and nuclear factor of activated T cells cytoplasmic 1 (NFATc1)). Furthermore, Cur-HD NPs markedly stimulated apoptosis of osteoclasts. Therefore, we hope that Cur-HD NPs will be useful nanodrugs for the treatment of bone-related diseases.

AB - Curcumin has various pharmacological activities such as anti-inflammatory, antioxidant, antimicrobial and antitumor activity. However, its use has been limited due to its poor solubility in water and minimal systemic bioavailability. Thus, we developed curcumin-delivering heparin-based nanoparticles and evaluated the inhibition effects of osteoclastogenesis by curcumin-delivering heparin nanoparticles (Cur-HD NPs). Cur-HD NPs were dispersed well in aqueous solution by forming self-assembled nanoparticles and showed sustained release of curcumin. In vitro studies, HD NPs facilitated intracellular delivery of curcumin into macrophages and osteoclasts, and thus, Cur-HD NPs effectively inhibited osteoclastogenesis in a dose-dependent manner by suppressing tartrate-resistant acid phosphatase (TRAP) activity and TRAP-positive multinucleated cells as well as by reducing the expression of osteoclast marker genes (i.e., TRAP and nuclear factor of activated T cells cytoplasmic 1 (NFATc1)). Furthermore, Cur-HD NPs markedly stimulated apoptosis of osteoclasts. Therefore, we hope that Cur-HD NPs will be useful nanodrugs for the treatment of bone-related diseases.

KW - RANKL

KW - curcumin

KW - heparin nanoparticles

KW - osteoclast

KW - osteoclastogenesis

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Enhanced effects of osteoclastogenesis inhibition by curcumin-delivering heparin nanoparticles

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Keywords

ASJC Scopus subject areas

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