Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection

Seonhwa Park; Ji Eun Jeong; Van Sang Le; Jeongwook Seo; Byeongjun Yu; Da Young Kim; Se Hun Kwon; Sangyong Jon; Han Young Woo; Haesik Yang

doi:10.1021/jacs.7b12382

Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection

Seonhwa Park, Ji Eun Jeong, Van Sang Le, Jeongwook Seo, Byeongjun Yu, Da Young Kim, Se Hun Kwon, Sangyong Jon, Han Young Woo, Haesik Yang

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Direct electron transfer between a redox label and an electrode requires a short working distance (<1-2 nm), and in general an affinity biosensor based on direct electron transfer requires a finely smoothed Au electrode to support efficient target binding. Here we report that direct electron transfer over a longer working distance is possible between (i) an anionic π-conjugated polyelectrolyte (CPE) label having many redox-active sites and (ii) a readily prepared, thin polymeric monolayer-modified indium-tin oxide electrode. In addition, the long CPE label (∼18 nm for 10 kDa) can approach the electrode within the working distance after sandwich-type target-specific binding, and fast CPE-mediated oxidation of ammonia borane along the entire CPE backbone affords high signal amplification.

Original language	English
Pages (from-to)	2409-2412
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	140
Issue number	7
DOIs	https://doi.org/10.1021/jacs.7b12382
Publication status	Published - 2018 Feb 21

Bibliographical note

Funding Information:
This work was supported by the Samsung Research Funding Center of Samsung Electronics (Project SRFC-TA1503-01) and the National Research Foundation of Korea (2017M3A7B4041973 and 2015R1D1A1A09056905).

Publisher Copyright:
© 2018 American Chemical Society.

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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title = "Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection",

abstract = "Direct electron transfer between a redox label and an electrode requires a short working distance (<1-2 nm), and in general an affinity biosensor based on direct electron transfer requires a finely smoothed Au electrode to support efficient target binding. Here we report that direct electron transfer over a longer working distance is possible between (i) an anionic π-conjugated polyelectrolyte (CPE) label having many redox-active sites and (ii) a readily prepared, thin polymeric monolayer-modified indium-tin oxide electrode. In addition, the long CPE label (∼18 nm for 10 kDa) can approach the electrode within the working distance after sandwich-type target-specific binding, and fast CPE-mediated oxidation of ammonia borane along the entire CPE backbone affords high signal amplification.",

author = "Seonhwa Park and Jeong, {Ji Eun} and Le, {Van Sang} and Jeongwook Seo and Byeongjun Yu and Kim, {Da Young} and Kwon, {Se Hun} and Sangyong Jon and Woo, {Han Young} and Haesik Yang",

note = "Funding Information: This work was supported by the Samsung Research Funding Center of Samsung Electronics (Project SRFC-TA1503-01) and the National Research Foundation of Korea (2017M3A7B4041973 and 2015R1D1A1A09056905). Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

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T1 - Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection

AU - Park, Seonhwa

AU - Jeong, Ji Eun

AU - Le, Van Sang

AU - Seo, Jeongwook

AU - Yu, Byeongjun

AU - Kim, Da Young

AU - Kwon, Se Hun

AU - Jon, Sangyong

AU - Woo, Han Young

AU - Yang, Haesik

N1 - Funding Information: This work was supported by the Samsung Research Funding Center of Samsung Electronics (Project SRFC-TA1503-01) and the National Research Foundation of Korea (2017M3A7B4041973 and 2015R1D1A1A09056905). Publisher Copyright: © 2018 American Chemical Society.

PY - 2018/2/21

Y1 - 2018/2/21

N2 - Direct electron transfer between a redox label and an electrode requires a short working distance (<1-2 nm), and in general an affinity biosensor based on direct electron transfer requires a finely smoothed Au electrode to support efficient target binding. Here we report that direct electron transfer over a longer working distance is possible between (i) an anionic π-conjugated polyelectrolyte (CPE) label having many redox-active sites and (ii) a readily prepared, thin polymeric monolayer-modified indium-tin oxide electrode. In addition, the long CPE label (∼18 nm for 10 kDa) can approach the electrode within the working distance after sandwich-type target-specific binding, and fast CPE-mediated oxidation of ammonia borane along the entire CPE backbone affords high signal amplification.

AB - Direct electron transfer between a redox label and an electrode requires a short working distance (<1-2 nm), and in general an affinity biosensor based on direct electron transfer requires a finely smoothed Au electrode to support efficient target binding. Here we report that direct electron transfer over a longer working distance is possible between (i) an anionic π-conjugated polyelectrolyte (CPE) label having many redox-active sites and (ii) a readily prepared, thin polymeric monolayer-modified indium-tin oxide electrode. In addition, the long CPE label (∼18 nm for 10 kDa) can approach the electrode within the working distance after sandwich-type target-specific binding, and fast CPE-mediated oxidation of ammonia borane along the entire CPE backbone affords high signal amplification.

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Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection

Abstract

Bibliographical note

ASJC Scopus subject areas

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