Enhanced production of cellobiose dehydrogenase and β-glucosidase by Phanerochaete chrysosporium

Eun Ji Kim; Han Suk Choi; Seong Woo Kang; Kwang Ho Song; Sung Ok Han; Chulhwan Park; Seung Wook Kim

doi:10.1007/s11814-011-0144-z

Enhanced production of cellobiose dehydrogenase and β-glucosidase by Phanerochaete chrysosporium

Eun Ji Kim, Han Suk Choi, Seong Woo Kang, Kwang Ho Song, Sung Ok Han, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The production of cellobiose dehydrogenase (CDH) and β-glucosidase by Phanerochaete chrysosporium ATCC 32629 was assessed during submerged fermentation. The maximum concentrations of CDH and β-glucosidase were obtained using rice straw as the carbon source. Organic nitrogen sources were more effective in enzyme production than inorganic nitrogen sources. Corn steep liquor (CSL) for CDH production and soy bean meal (SBM) for β-glucosidase production were the most appropriate organic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentrations of CDH and β-glucosidase achieved in the stirred-tank reactor (STR) were 204 U/L and 140 U/L, respectively. CDH productivity (22.7 U/L·day) was the highest at 9 days.

Original language	English
Pages (from-to)	77-81
Number of pages	5
Journal	Korean Journal of Chemical Engineering
Volume	29
Issue number	1
DOIs	https://doi.org/10.1007/s11814-011-0144-z
Publication status	Published - 2012 Jan

Bibliographical note

Funding Information:
of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2010-0027563).

Funding Information:
This work was supported by the National Research Foundation

Keywords

Cellobiose Dehydrogenase
Phanerochaete chrysosporium
Response Surface Method
Rice Straw
β-Glucosidase

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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10.1007/s11814-011-0144-z

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title = "Enhanced production of cellobiose dehydrogenase and β-glucosidase by Phanerochaete chrysosporium",

abstract = "The production of cellobiose dehydrogenase (CDH) and β-glucosidase by Phanerochaete chrysosporium ATCC 32629 was assessed during submerged fermentation. The maximum concentrations of CDH and β-glucosidase were obtained using rice straw as the carbon source. Organic nitrogen sources were more effective in enzyme production than inorganic nitrogen sources. Corn steep liquor (CSL) for CDH production and soy bean meal (SBM) for β-glucosidase production were the most appropriate organic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentrations of CDH and β-glucosidase achieved in the stirred-tank reactor (STR) were 204 U/L and 140 U/L, respectively. CDH productivity (22.7 U/L·day) was the highest at 9 days.",

keywords = "Cellobiose Dehydrogenase, Phanerochaete chrysosporium, Response Surface Method, Rice Straw, β-Glucosidase",

author = "Kim, {Eun Ji} and Choi, {Han Suk} and Kang, {Seong Woo} and Song, {Kwang Ho} and Han, {Sung Ok} and Chulhwan Park and Kim, {Seung Wook}",

note = "Funding Information: of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2010-0027563). Funding Information: This work was supported by the National Research Foundation",

year = "2012",

month = jan,

doi = "10.1007/s11814-011-0144-z",

language = "English",

volume = "29",

pages = "77--81",

journal = "Korean Journal of Chemical Engineering",

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T1 - Enhanced production of cellobiose dehydrogenase and β-glucosidase by Phanerochaete chrysosporium

AU - Kim, Eun Ji

AU - Choi, Han Suk

AU - Kang, Seong Woo

AU - Song, Kwang Ho

AU - Han, Sung Ok

AU - Park, Chulhwan

AU - Kim, Seung Wook

N1 - Funding Information: of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2010-0027563). Funding Information: This work was supported by the National Research Foundation

PY - 2012/1

Y1 - 2012/1

N2 - The production of cellobiose dehydrogenase (CDH) and β-glucosidase by Phanerochaete chrysosporium ATCC 32629 was assessed during submerged fermentation. The maximum concentrations of CDH and β-glucosidase were obtained using rice straw as the carbon source. Organic nitrogen sources were more effective in enzyme production than inorganic nitrogen sources. Corn steep liquor (CSL) for CDH production and soy bean meal (SBM) for β-glucosidase production were the most appropriate organic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentrations of CDH and β-glucosidase achieved in the stirred-tank reactor (STR) were 204 U/L and 140 U/L, respectively. CDH productivity (22.7 U/L·day) was the highest at 9 days.

AB - The production of cellobiose dehydrogenase (CDH) and β-glucosidase by Phanerochaete chrysosporium ATCC 32629 was assessed during submerged fermentation. The maximum concentrations of CDH and β-glucosidase were obtained using rice straw as the carbon source. Organic nitrogen sources were more effective in enzyme production than inorganic nitrogen sources. Corn steep liquor (CSL) for CDH production and soy bean meal (SBM) for β-glucosidase production were the most appropriate organic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentrations of CDH and β-glucosidase achieved in the stirred-tank reactor (STR) were 204 U/L and 140 U/L, respectively. CDH productivity (22.7 U/L·day) was the highest at 9 days.

KW - Cellobiose Dehydrogenase

KW - Phanerochaete chrysosporium

KW - Response Surface Method

KW - Rice Straw

KW - β-Glucosidase

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DO - 10.1007/s11814-011-0144-z

M3 - Article

AN - SCOPUS:84855302600

SN - 0256-1115

VL - 29

SP - 77

EP - 81

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

IS - 1

ER -

Enhanced production of cellobiose dehydrogenase and β-glucosidase by Phanerochaete chrysosporium

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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