TY - JOUR
T1 - Enhanced resolution of a surface plasmon resonance sensor detecting C-reactive protein via a bimetallic waveguide-coupled mode approach
AU - Lee, Hwa Seub
AU - Seong, Tae Yeon
AU - Kim, Won Mok
AU - Kim, Inho
AU - Hwang, Gyu Weon
AU - Lee, Wook Seong
AU - Lee, Kyeong Seok
N1 - Funding Information:
This work was supported by an institutional program grant ( 2E27150 ) from the Korea Institute of Science and Technology . This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea ( 20163010012580 ).
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Extensive efforts have been devoted to improving the resolution of surface plasmon resonance (SPR) sensors in the detection of trace levels of biomolecules considered essential for the early diagnosis of disease. As part of these efforts, we previously developed a bimetallic waveguide-coupled surface plasmon resonance (Bi-WCSPR) sensor configuration designed to dramatically reduce the linewidth of the SPR curve while preserving a moderate local field decay length, thereby addressing the resolution issue. In this study, we systematically quantified the enhanced resolution in Bi-WCSPR sensors used to detect C-reactive protein (CRP), a widely accepted biomarker of inflammation in cardiovascular disease. Individual SPR sensorgrams were measured at several CRP concentrations in both the intensity and angular interrogation modes, confirming that the Bi-WCSPR configuration with a high Ag-to-Au ratio provided a figure-of-merit (FOM) that was 2.53 times higher than that of conventional Au sensors and a limit-of-detection (LOD) that was an order of magnitude lower than the cut-off level for CRP. It was found that when using the Bi-WCSPR configurations, the resolution enhancement in the intensity interrogation mode becomes more evident as the CRP concentrations is lowered to the cut-off level.
AB - Extensive efforts have been devoted to improving the resolution of surface plasmon resonance (SPR) sensors in the detection of trace levels of biomolecules considered essential for the early diagnosis of disease. As part of these efforts, we previously developed a bimetallic waveguide-coupled surface plasmon resonance (Bi-WCSPR) sensor configuration designed to dramatically reduce the linewidth of the SPR curve while preserving a moderate local field decay length, thereby addressing the resolution issue. In this study, we systematically quantified the enhanced resolution in Bi-WCSPR sensors used to detect C-reactive protein (CRP), a widely accepted biomarker of inflammation in cardiovascular disease. Individual SPR sensorgrams were measured at several CRP concentrations in both the intensity and angular interrogation modes, confirming that the Bi-WCSPR configuration with a high Ag-to-Au ratio provided a figure-of-merit (FOM) that was 2.53 times higher than that of conventional Au sensors and a limit-of-detection (LOD) that was an order of magnitude lower than the cut-off level for CRP. It was found that when using the Bi-WCSPR configurations, the resolution enhancement in the intensity interrogation mode becomes more evident as the CRP concentrations is lowered to the cut-off level.
KW - Bimetallic waveguide-coupled surface plasmon resonance
KW - C-reactive protein
KW - Resolution enhancement
KW - Surface plasmon resonance sensor
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U2 - 10.1016/j.snb.2018.03.136
DO - 10.1016/j.snb.2018.03.136
M3 - Article
AN - SCOPUS:85053783438
SN - 0925-4005
VL - 266
SP - 311
EP - 317
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -