Abstract
We developed magnetically-separable and highly-stable biocatalyst system of Mugil cephalus epoxide hydrolase (McEH) for enantioselective hydrolysis of racemic epoxides. McEH protein was adsorbed and cross-linked into mesoporous silica with bottle-neck mesopores, which effectively prevented the leaching of cross-linked McEH in a larger mesocellular pores. This ship-in-a-bottle approach allows for highly loaded and stable McEH system. For example, free McEH showed only 7.5% of initial activity under shaking condition at 80 h while ship-in-a-bottled McEH retained 79.5% of initial activity in the same condition. Stable McEH in magnetically-separable mesoporous silica could achieve the yield of 45% for the preparation of enantiopure (S)-styrene oxide with 98%ee. The magnetic nanoparticles pre-incorporated in mesoporous silica enabled an easy recovery of immobilized McEH for repetitive batch resolutions of racemic styrene oxide, allowing for more than 50% of the initial activity was retained after seven recycled uses.
Original language | English |
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Pages (from-to) | 48-51 |
Number of pages | 4 |
Journal | Journal of Molecular Catalysis B: Enzymatic |
Volume | 89 |
DOIs | |
Publication status | Published - 2013 May |
Bibliographical note
Funding Information:This work was supported by the Marine and Extreme Genome Research Center Program , Ministry of Land , Transportation and Maritime Affairs, Republic of Korea . This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2012K001385 ).
Keywords
- Enzyme immobilization
- Epoxide hydrolase
- Mesoporous silica
- Mugil cephalus
- Nanoscale enzyme reactor
ASJC Scopus subject areas
- Catalysis
- Bioengineering
- Biochemistry
- Process Chemistry and Technology