TY - JOUR
T1 - Enhanced th1 immune responses induced with an ova-il-12 fusion protein
AU - Kim, T. S.
AU - Dekruyff, R. H.
AU - Maecker, H. T.
AU - Levy, S.
AU - Umetsu, D. T.
PY - 1996
Y1 - 1996
N2 - The form of immunity that occurs during a particular immune response is dependent on the development of T cells producing specific, restricted cytokine profiles. In order to direct the form of the immune response in an antigen-specific manner, we constructed a cytokine fusion protein (OVA-IL12) that contained the T cell-dependent antigen ovalbumin (OVA), fused to murine IL-12. The OVA-IL-12 fusion protein was produced in a baculoyirus expression system and was purified by anti-OVA immunqaffinity chromatography. The purified OVA-IL-12 protein was characterized by SDS-PAGE and by Western blot analysis using anti-OVA and anti-p40 antibodies, and displayed potent IL-12 bioactivity in an IL-12 proliferation assay. BALB/c mice immunized with the OVA-IL-12 fusion protein produced increased quantities of anti-OVA lgG2a, but decreased anti-OVA-lgG1 antibody compared with mice immunized with recombinant OVA alone. Spleen and lymph node cells from the immunized mice produced large amounts of IFN-y when restimulated in vitro with OVA, while those from mice immunized with the OVA alone or from non immunized mice produced little or no IFN-y. In contrast, immunization with OVA and free rlL-12 enhanced T cell production of IFN-y, but the IFN-y production was not antigen-specific. Moreover, the OVA-IL-12 protein was effective in enhancing IFN-y production in OVA-primed mice. BALB/c mice previously injected with OVA in alum and subsequently immunized with the OVA-IL-12 protein developed a significant increase in antigen-specific IFN-y production and a significant decrease in antigen-specific IL-4 production. These studies indicate that the OVA-IL-12 fusion protein can induce OVA-specific Th1 responses, and that the fusion protein confines the effect of IL-12 to OVA-specific T cells.
AB - The form of immunity that occurs during a particular immune response is dependent on the development of T cells producing specific, restricted cytokine profiles. In order to direct the form of the immune response in an antigen-specific manner, we constructed a cytokine fusion protein (OVA-IL12) that contained the T cell-dependent antigen ovalbumin (OVA), fused to murine IL-12. The OVA-IL-12 fusion protein was produced in a baculoyirus expression system and was purified by anti-OVA immunqaffinity chromatography. The purified OVA-IL-12 protein was characterized by SDS-PAGE and by Western blot analysis using anti-OVA and anti-p40 antibodies, and displayed potent IL-12 bioactivity in an IL-12 proliferation assay. BALB/c mice immunized with the OVA-IL-12 fusion protein produced increased quantities of anti-OVA lgG2a, but decreased anti-OVA-lgG1 antibody compared with mice immunized with recombinant OVA alone. Spleen and lymph node cells from the immunized mice produced large amounts of IFN-y when restimulated in vitro with OVA, while those from mice immunized with the OVA alone or from non immunized mice produced little or no IFN-y. In contrast, immunization with OVA and free rlL-12 enhanced T cell production of IFN-y, but the IFN-y production was not antigen-specific. Moreover, the OVA-IL-12 protein was effective in enhancing IFN-y production in OVA-primed mice. BALB/c mice previously injected with OVA in alum and subsequently immunized with the OVA-IL-12 protein developed a significant increase in antigen-specific IFN-y production and a significant decrease in antigen-specific IL-4 production. These studies indicate that the OVA-IL-12 fusion protein can induce OVA-specific Th1 responses, and that the fusion protein confines the effect of IL-12 to OVA-specific T cells.
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M3 - Article
AN - SCOPUS:33749151195
SN - 1530-6860
VL - 10
SP - A1445
JO - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
JF - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
IS - 6
ER -