Abstract
A soil biosensor was developed in our previous study using both a recombinant bioluminescent bacterium, GC2 (lac::luxCDABE), immobilized with transparent glass beads within an agar matrix and rhamnolipids, a biosurfactant employed to increase the bioavailability of phenanthrene from contaminated soil. To improve the sensitivity of this biosensor, glass beads of various sizes were tested. The results showed that the detection limit of the biosensor was lowered when smaller-sized glass beads were employed. Further tests showed that the use of glass beads with smaller diameters enhanced the sensitivity of the biosensor, allowing for a more conclusive study on the phenanthrene toxicity present within soils.
Original language | English |
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Pages (from-to) | 272-276 |
Number of pages | 5 |
Journal | Sensors and Actuators, B: Chemical |
Volume | 97 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - 2004 Feb 1 |
Externally published | Yes |
Keywords
- Bioluminescent bacteria
- Biosensor
- Enhancement
- PAH
- Sensitivity
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry