Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng

Seung Hyun Kim; Seung Sik Cho; Jaya Ram Simkhada; Hyo Jeong Lee; Si Wouk Kim; Tae Sung Kim; Jin Cheol Yoo

doi:10.1271/bbb.80823

Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng

Seung Hyun Kim, Seung Sik Cho, Jaya Ram Simkhada, Hyo Jeong Lee, Si Wouk Kim, Tae Sung Kim, Jin Cheol Yoo

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Ginseng (Panax ginseng C.A. Meyer) has a wide range of therapeutic uses including cancer treatment. Human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)₂D₃] or all-trans retinoic acid (ATRA). Treatment of HL-60 cells with zero to 100μg/ml of a methanol extract of ginseng for 72 h induced a small increase in cell differentiation. Surprisingly, a synergistic induction of differentiation was observed when HL-60 cells were treated with ATRA or 1,25-(OH)₂D₃ and the extract. The inhibitors of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K), inhibited the HL-60 differentiation induced by the extract in combination with ATRA or 1,25-(OH)₂D₃, signifying that PKC and ERK were involved in the cell differentiation enhancement by the extract. These results suggest that the ability of a methanol extract of ginseng to enhance the differentiation potential of ATRA or 1,25-(OH)₂D₃ may improve the ultimate outcome of acute promyelocytic leukemia therapy.

Original language	English
Pages (from-to)	1048-1053
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	73
Issue number	5
DOIs	https://doi.org/10.1271/bbb.80823
Publication status	Published - 2009

Bibliographical note

Funding Information:
This work was supported by grant no. RTI-04-03-03 (BPF-2004-S-1-5(A)) from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE).

Keywords

1,25-dihydroxyvitamin D
All-trans retinoic acid
HL-60 cells
Leukemia
Panax ginseng

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1271/bbb.80823

Cite this

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title = "Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng",

abstract = "Ginseng (Panax ginseng C.A. Meyer) has a wide range of therapeutic uses including cancer treatment. Human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] or all-trans retinoic acid (ATRA). Treatment of HL-60 cells with zero to 100μg/ml of a methanol extract of ginseng for 72 h induced a small increase in cell differentiation. Surprisingly, a synergistic induction of differentiation was observed when HL-60 cells were treated with ATRA or 1,25-(OH)2D3 and the extract. The inhibitors of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K), inhibited the HL-60 differentiation induced by the extract in combination with ATRA or 1,25-(OH)2D3, signifying that PKC and ERK were involved in the cell differentiation enhancement by the extract. These results suggest that the ability of a methanol extract of ginseng to enhance the differentiation potential of ATRA or 1,25-(OH)2D3 may improve the ultimate outcome of acute promyelocytic leukemia therapy.",

keywords = "1,25-dihydroxyvitamin D, All-trans retinoic acid, HL-60 cells, Leukemia, Panax ginseng",

author = "Kim, {Seung Hyun} and Cho, {Seung Sik} and Simkhada, {Jaya Ram} and Lee, {Hyo Jeong} and Kim, {Si Wouk} and Kim, {Tae Sung} and Yoo, {Jin Cheol}",

note = "Funding Information: This work was supported by grant no. RTI-04-03-03 (BPF-2004-S-1-5(A)) from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE).",

year = "2009",

doi = "10.1271/bbb.80823",

language = "English",

volume = "73",

pages = "1048--1053",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "5",

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T1 - Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng

AU - Kim, Seung Hyun

AU - Cho, Seung Sik

AU - Simkhada, Jaya Ram

AU - Lee, Hyo Jeong

AU - Kim, Si Wouk

AU - Kim, Tae Sung

AU - Yoo, Jin Cheol

N1 - Funding Information: This work was supported by grant no. RTI-04-03-03 (BPF-2004-S-1-5(A)) from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE).

PY - 2009

Y1 - 2009

N2 - Ginseng (Panax ginseng C.A. Meyer) has a wide range of therapeutic uses including cancer treatment. Human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] or all-trans retinoic acid (ATRA). Treatment of HL-60 cells with zero to 100μg/ml of a methanol extract of ginseng for 72 h induced a small increase in cell differentiation. Surprisingly, a synergistic induction of differentiation was observed when HL-60 cells were treated with ATRA or 1,25-(OH)2D3 and the extract. The inhibitors of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K), inhibited the HL-60 differentiation induced by the extract in combination with ATRA or 1,25-(OH)2D3, signifying that PKC and ERK were involved in the cell differentiation enhancement by the extract. These results suggest that the ability of a methanol extract of ginseng to enhance the differentiation potential of ATRA or 1,25-(OH)2D3 may improve the ultimate outcome of acute promyelocytic leukemia therapy.

AB - Ginseng (Panax ginseng C.A. Meyer) has a wide range of therapeutic uses including cancer treatment. Human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] or all-trans retinoic acid (ATRA). Treatment of HL-60 cells with zero to 100μg/ml of a methanol extract of ginseng for 72 h induced a small increase in cell differentiation. Surprisingly, a synergistic induction of differentiation was observed when HL-60 cells were treated with ATRA or 1,25-(OH)2D3 and the extract. The inhibitors of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K), inhibited the HL-60 differentiation induced by the extract in combination with ATRA or 1,25-(OH)2D3, signifying that PKC and ERK were involved in the cell differentiation enhancement by the extract. These results suggest that the ability of a methanol extract of ginseng to enhance the differentiation potential of ATRA or 1,25-(OH)2D3 may improve the ultimate outcome of acute promyelocytic leukemia therapy.

KW - 1,25-dihydroxyvitamin D

KW - All-trans retinoic acid

KW - HL-60 cells

KW - Leukemia

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Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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