Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b

Jeewon Lee; Bhupendra K. Soni; Robert L. Kelley

doi:10.1007/BF00154617

Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b

Jeewon Lee, Bhupendra K. Soni, Robert L. Kelley

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12 g/L in continuous culture and biomass productivity was enhanced up to 0.708 g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5 mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.

Original language	English
Pages (from-to)	897-902
Number of pages	6
Journal	Biotechnology letters
Volume	18
Issue number	8
DOIs	https://doi.org/10.1007/BF00154617
Publication status	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b",

abstract = "Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12 g/L in continuous culture and biomass productivity was enhanced up to 0.708 g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5 mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.",

author = "Jeewon Lee and Soni, {Bhupendra K.} and Kelley, {Robert L.}",

year = "1996",

doi = "10.1007/BF00154617",

language = "English",

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pages = "897--902",

journal = "Biotechnology Letters",

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T1 - Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b

AU - Lee, Jeewon

AU - Soni, Bhupendra K.

AU - Kelley, Robert L.

PY - 1996

Y1 - 1996

N2 - Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12 g/L in continuous culture and biomass productivity was enhanced up to 0.708 g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5 mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.

AB - Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12 g/L in continuous culture and biomass productivity was enhanced up to 0.708 g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5 mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.

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ER -

Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b

Abstract

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