Abstract
Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12 g/L in continuous culture and biomass productivity was enhanced up to 0.708 g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5 mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.
Original language | English |
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Pages (from-to) | 897-902 |
Number of pages | 6 |
Journal | Biotechnology letters |
Volume | 18 |
Issue number | 8 |
DOIs | |
Publication status | Published - 1996 |
Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology