Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485

Min Seo Jeon; Jeong Joo Oh; Jee Young Kim; Sang Il Han; Sang Jun Sim; Yoon E. Choi

doi:10.1016/j.biortech.2019.121513

Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485

Min Seo Jeon, Jeong Joo Oh, Jee Young Kim, Sang Il Han, Sang Jun Sim, Yoon E. Choi

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

This study investigated the putative effects of co-cultivation of Euglena gracilis with Pseudoalteromonas sp. MEBiC 03485 on the growth of E. gracilis and its paramylon production. The strain MEBiC 03485 had beneficial effects on the growth and paramylon contents of E. gracilis. To determine the optimal conditions for co-cultivation, the effects of algal to bacterial inoculum ratios and E. gracilis growth stages were examined. Under optimal conditions, the biomass productivity and paramylon production were increased by more than 23% and 34%, respectively. These effects were attributed to the extracellular polymeric substances (EPS) from the strain MEBiC 03485. GC–MS and HPAEC were carried out to analyze the composition of EPS. It was found that the EPS consisted of rhamnose, galactose, glucose, and mannose. These results suggest a novel approach for potentially enhancing the growth of E. gracilis as well as its paramylon production, via co-culturing with the symbiotic strain MEBiC 03485.

Original language	English
Article number	121513
Journal	Bioresource technology
Volume	288
DOIs	https://doi.org/10.1016/j.biortech.2019.121513
Publication status	Published - 2019 Sept

Bibliographical note

Funding Information:
This research was supported by a grant from the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST), which is funded by the Ministry of Oceans and Fisheries (MOF) (No. 20170488 ). This research was supported by the KAERI research program and the National Research Foundation of Korea (NRF), which is funded by the Korea government (MSIP) ( 2016R1D1A1B03932773 and 2018M3A9F3055925 ).

Funding Information:
This research was supported by a grant from the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST), which is funded by the Ministry of Oceans and Fisheries (MOF) (No.20170488). This research was supported by the KAERI research program and the National Research Foundation of Korea (NRF), which is funded by the Korea government (MSIP) (2016R1D1A1B03932773 and 2018M3A9F3055925).

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Co-cultivation
Euglena gracilis
Extracellular polymeric substances
Paramylon
Pseudoalteromonas

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.121513

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title = "Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485",

abstract = "This study investigated the putative effects of co-cultivation of Euglena gracilis with Pseudoalteromonas sp. MEBiC 03485 on the growth of E. gracilis and its paramylon production. The strain MEBiC 03485 had beneficial effects on the growth and paramylon contents of E. gracilis. To determine the optimal conditions for co-cultivation, the effects of algal to bacterial inoculum ratios and E. gracilis growth stages were examined. Under optimal conditions, the biomass productivity and paramylon production were increased by more than 23% and 34%, respectively. These effects were attributed to the extracellular polymeric substances (EPS) from the strain MEBiC 03485. GC–MS and HPAEC were carried out to analyze the composition of EPS. It was found that the EPS consisted of rhamnose, galactose, glucose, and mannose. These results suggest a novel approach for potentially enhancing the growth of E. gracilis as well as its paramylon production, via co-culturing with the symbiotic strain MEBiC 03485.",

keywords = "Co-cultivation, Euglena gracilis, Extracellular polymeric substances, Paramylon, Pseudoalteromonas",

author = "Jeon, {Min Seo} and Oh, {Jeong Joo} and Kim, {Jee Young} and Han, {Sang Il} and Sim, {Sang Jun} and Choi, {Yoon E.}",

note = "Funding Information: This research was supported by a grant from the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST), which is funded by the Ministry of Oceans and Fisheries (MOF) (No. 20170488 ). This research was supported by the KAERI research program and the National Research Foundation of Korea (NRF), which is funded by the Korea government (MSIP) ( 2016R1D1A1B03932773 and 2018M3A9F3055925 ). Funding Information: This research was supported by a grant from the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST), which is funded by the Ministry of Oceans and Fisheries (MOF) (No.20170488). This research was supported by the KAERI research program and the National Research Foundation of Korea (NRF), which is funded by the Korea government (MSIP) (2016R1D1A1B03932773 and 2018M3A9F3055925). Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

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AU - Oh, Jeong Joo

AU - Kim, Jee Young

AU - Han, Sang Il

AU - Sim, Sang Jun

AU - Choi, Yoon E.

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PY - 2019/9

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N2 - This study investigated the putative effects of co-cultivation of Euglena gracilis with Pseudoalteromonas sp. MEBiC 03485 on the growth of E. gracilis and its paramylon production. The strain MEBiC 03485 had beneficial effects on the growth and paramylon contents of E. gracilis. To determine the optimal conditions for co-cultivation, the effects of algal to bacterial inoculum ratios and E. gracilis growth stages were examined. Under optimal conditions, the biomass productivity and paramylon production were increased by more than 23% and 34%, respectively. These effects were attributed to the extracellular polymeric substances (EPS) from the strain MEBiC 03485. GC–MS and HPAEC were carried out to analyze the composition of EPS. It was found that the EPS consisted of rhamnose, galactose, glucose, and mannose. These results suggest a novel approach for potentially enhancing the growth of E. gracilis as well as its paramylon production, via co-culturing with the symbiotic strain MEBiC 03485.

AB - This study investigated the putative effects of co-cultivation of Euglena gracilis with Pseudoalteromonas sp. MEBiC 03485 on the growth of E. gracilis and its paramylon production. The strain MEBiC 03485 had beneficial effects on the growth and paramylon contents of E. gracilis. To determine the optimal conditions for co-cultivation, the effects of algal to bacterial inoculum ratios and E. gracilis growth stages were examined. Under optimal conditions, the biomass productivity and paramylon production were increased by more than 23% and 34%, respectively. These effects were attributed to the extracellular polymeric substances (EPS) from the strain MEBiC 03485. GC–MS and HPAEC were carried out to analyze the composition of EPS. It was found that the EPS consisted of rhamnose, galactose, glucose, and mannose. These results suggest a novel approach for potentially enhancing the growth of E. gracilis as well as its paramylon production, via co-culturing with the symbiotic strain MEBiC 03485.

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KW - Paramylon

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Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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