Abstract
Genetic manipulation of the Porphyridium sp. may increase the production of phycoerythrin. Since phycobiliproteins capture and transfer energy to both photosystems (PS I and PS II), it was hypothesized that the gene mutation involved increases phycoerythrin synthesis. The gene encoding chlorophyll synthase (CHS1) was selected as chlorophyll synthase plays an important role in photosynthesis, mediating the final process of chlorophyll synthesis. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 ribonucleoprotein (CRISPR/Cas9 RNP) delivery system was used to generate the chlorophyll synthase loss-of-function mutants (Δchs1). Independent Δchs1 showed no differences in the growth and production of sulfated polysaccharide compared to control. Phycoerythrin contents of the two independent mutants substantially increased regardless of light source. This study provides a novel applicability for the CRISPR/Cas9 RNP method in red microalgae toward a bio-product of interest. The obtained mutants could serve as potential producers of phycoerythrin if Porphyridium is selected as a natural source.
Original language | English |
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Article number | 124974 |
Journal | Bioresource technology |
Volume | 330 |
DOIs | |
Publication status | Published - 2021 Jun |
Bibliographical note
Funding Information:This research was supported by Korea University Future Research Grant and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2019R1A2C2087449). This work was also supported by Regional Specialized Industry Development Program funded by the Ministry of SMEs and Startups (S2933804).
Publisher Copyright:
© 2021
Keywords
- CRISPR/Cas9 RNP method
- Chlorophyll
- Phycoerythrin
- Porphyridium sp.
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal