Enhancement of phycoerythrin productivity in Porphyridium purpureum using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 ribonucleoprotein system

Min Seo Jeon, Sang Il Han, Minsol Jeon, Yoon E. Choi

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    Genetic manipulation of the Porphyridium sp. may increase the production of phycoerythrin. Since phycobiliproteins capture and transfer energy to both photosystems (PS I and PS II), it was hypothesized that the gene mutation involved increases phycoerythrin synthesis. The gene encoding chlorophyll synthase (CHS1) was selected as chlorophyll synthase plays an important role in photosynthesis, mediating the final process of chlorophyll synthesis. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 ribonucleoprotein (CRISPR/Cas9 RNP) delivery system was used to generate the chlorophyll synthase loss-of-function mutants (Δchs1). Independent Δchs1 showed no differences in the growth and production of sulfated polysaccharide compared to control. Phycoerythrin contents of the two independent mutants substantially increased regardless of light source. This study provides a novel applicability for the CRISPR/Cas9 RNP method in red microalgae toward a bio-product of interest. The obtained mutants could serve as potential producers of phycoerythrin if Porphyridium is selected as a natural source.

    Original languageEnglish
    Article number124974
    JournalBioresource technology
    Volume330
    DOIs
    Publication statusPublished - 2021 Jun

    Bibliographical note

    Funding Information:
    This research was supported by Korea University Future Research Grant and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2019R1A2C2087449). This work was also supported by Regional Specialized Industry Development Program funded by the Ministry of SMEs and Startups (S2933804).

    Publisher Copyright:
    © 2021

    Keywords

    • CRISPR/Cas9 RNP method
    • Chlorophyll
    • Phycoerythrin
    • Porphyridium sp.

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Renewable Energy, Sustainability and the Environment
    • Waste Management and Disposal

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