TY - JOUR
T1 - Enhancement of the multi-channel continuous monitoring system through the use of Xenorhabdus luminescens lux fusions
AU - Lee, Jin Hyung
AU - Mitchell, Robert J.
AU - Gu, Man Bock
N1 - Funding Information:
The authors would like to thank Dr. Robert LaRossa for E. Coli strains RFM443 and DPD2511 (RFM443—pkatG::V. fischeri luxCDABE) and plasmid pDEW201, which were used in this study. Robert would also like to thank the Lord Jesus for giving him the chance to do work that he loves. This research was supported by Korea Science and Engineering Foundation (KOSEF) through Advanced Environmental Monitoring Research Center (ADEMRC) in Kwangju Institute of Science and Technology (K-JIST). We are grateful for the support.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/10/15
Y1 - 2004/10/15
N2 - The enhancement of the multi-channel continuous toxicity monitoring system developed previously was studied. To achieve better and more stable results from the system, the use of thermo-lux fusion strains that express the luxCDABE genes from Xenorhabdus luminescens was evaluated. A total of six recombinant Escherichia coli strains with the promoters from three oxidative-stress responsive genes, i.e. the katG, sodA and pqi-5 genes, fused to either the lux genes from Vibrio fischeri or X. luminescens were characterized and their responses to different chemicals compared. It was found that the basal level bioluminescence (BL) from the thermo-lux fusion strains was always higher while that of the V. fischeri lux strains were always near or below the lower limit of detection of the system. For example, the katG::V. fischeri lux strain, DPD2511, gave no discernible response due to its low level expression while a fusion of the katG promoter with the X. luminescens lux operon was clearly responsive and capable of detecting hydrogen peroxide down to about 1 ppm. The use of the thermo-lux strains found them to be as sensitive as the V. fischeri lux strains while providing a brighter, more stable basal level bioluminescence, making the analysis and monitoring of water-borne toxicity more reliable.
AB - The enhancement of the multi-channel continuous toxicity monitoring system developed previously was studied. To achieve better and more stable results from the system, the use of thermo-lux fusion strains that express the luxCDABE genes from Xenorhabdus luminescens was evaluated. A total of six recombinant Escherichia coli strains with the promoters from three oxidative-stress responsive genes, i.e. the katG, sodA and pqi-5 genes, fused to either the lux genes from Vibrio fischeri or X. luminescens were characterized and their responses to different chemicals compared. It was found that the basal level bioluminescence (BL) from the thermo-lux fusion strains was always higher while that of the V. fischeri lux strains were always near or below the lower limit of detection of the system. For example, the katG::V. fischeri lux strain, DPD2511, gave no discernible response due to its low level expression while a fusion of the katG promoter with the X. luminescens lux operon was clearly responsive and capable of detecting hydrogen peroxide down to about 1 ppm. The use of the thermo-lux strains found them to be as sensitive as the V. fischeri lux strains while providing a brighter, more stable basal level bioluminescence, making the analysis and monitoring of water-borne toxicity more reliable.
KW - Bioluminescence
KW - Multi-channel continuous monitoring system
KW - Oxidative stress
KW - Thermo-lux strains
KW - Xenorhabdus luminescens
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U2 - 10.1016/j.bios.2004.02.019
DO - 10.1016/j.bios.2004.02.019
M3 - Article
C2 - 15494228
AN - SCOPUS:7444235422
SN - 0956-5663
VL - 20
SP - 475
EP - 481
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 3
ER -