Enhancement of the multi-channel continuous monitoring system through the use of Xenorhabdus luminescens lux fusions

Jin Hyung Lee; Robert J. Mitchell; Man Bock Gu

doi:10.1016/j.bios.2004.02.019

Enhancement of the multi-channel continuous monitoring system through the use of Xenorhabdus luminescens lux fusions

Jin Hyung Lee, Robert J. Mitchell, Man Bock Gu

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The enhancement of the multi-channel continuous toxicity monitoring system developed previously was studied. To achieve better and more stable results from the system, the use of thermo-lux fusion strains that express the luxCDABE genes from Xenorhabdus luminescens was evaluated. A total of six recombinant Escherichia coli strains with the promoters from three oxidative-stress responsive genes, i.e. the katG, sodA and pqi-5 genes, fused to either the lux genes from Vibrio fischeri or X. luminescens were characterized and their responses to different chemicals compared. It was found that the basal level bioluminescence (BL) from the thermo-lux fusion strains was always higher while that of the V. fischeri lux strains were always near or below the lower limit of detection of the system. For example, the katG::V. fischeri lux strain, DPD2511, gave no discernible response due to its low level expression while a fusion of the katG promoter with the X. luminescens lux operon was clearly responsive and capable of detecting hydrogen peroxide down to about 1 ppm. The use of the thermo-lux strains found them to be as sensitive as the V. fischeri lux strains while providing a brighter, more stable basal level bioluminescence, making the analysis and monitoring of water-borne toxicity more reliable.

Original language	English
Pages (from-to)	475-481
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	20
Issue number	3
DOIs	https://doi.org/10.1016/j.bios.2004.02.019
Publication status	Published - 2004 Oct 15
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Dr. Robert LaRossa for E. Coli strains RFM443 and DPD2511 (RFM443—pkatG::V. fischeri luxCDABE) and plasmid pDEW201, which were used in this study. Robert would also like to thank the Lord Jesus for giving him the chance to do work that he loves. This research was supported by Korea Science and Engineering Foundation (KOSEF) through Advanced Environmental Monitoring Research Center (ADEMRC) in Kwangju Institute of Science and Technology (K-JIST). We are grateful for the support.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

Bioluminescence
Multi-channel continuous monitoring system
Oxidative stress
Thermo-lux strains
Xenorhabdus luminescens

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2004.02.019

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title = "Enhancement of the multi-channel continuous monitoring system through the use of Xenorhabdus luminescens lux fusions",

abstract = "The enhancement of the multi-channel continuous toxicity monitoring system developed previously was studied. To achieve better and more stable results from the system, the use of thermo-lux fusion strains that express the luxCDABE genes from Xenorhabdus luminescens was evaluated. A total of six recombinant Escherichia coli strains with the promoters from three oxidative-stress responsive genes, i.e. the katG, sodA and pqi-5 genes, fused to either the lux genes from Vibrio fischeri or X. luminescens were characterized and their responses to different chemicals compared. It was found that the basal level bioluminescence (BL) from the thermo-lux fusion strains was always higher while that of the V. fischeri lux strains were always near or below the lower limit of detection of the system. For example, the katG::V. fischeri lux strain, DPD2511, gave no discernible response due to its low level expression while a fusion of the katG promoter with the X. luminescens lux operon was clearly responsive and capable of detecting hydrogen peroxide down to about 1 ppm. The use of the thermo-lux strains found them to be as sensitive as the V. fischeri lux strains while providing a brighter, more stable basal level bioluminescence, making the analysis and monitoring of water-borne toxicity more reliable.",

keywords = "Bioluminescence, Multi-channel continuous monitoring system, Oxidative stress, Thermo-lux strains, Xenorhabdus luminescens",

author = "Lee, {Jin Hyung} and Mitchell, {Robert J.} and Gu, {Man Bock}",

note = "Funding Information: The authors would like to thank Dr. Robert LaRossa for E. Coli strains RFM443 and DPD2511 (RFM443—pkatG::V. fischeri luxCDABE) and plasmid pDEW201, which were used in this study. Robert would also like to thank the Lord Jesus for giving him the chance to do work that he loves. This research was supported by Korea Science and Engineering Foundation (KOSEF) through Advanced Environmental Monitoring Research Center (ADEMRC) in Kwangju Institute of Science and Technology (K-JIST). We are grateful for the support. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2004",

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doi = "10.1016/j.bios.2004.02.019",

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N2 - The enhancement of the multi-channel continuous toxicity monitoring system developed previously was studied. To achieve better and more stable results from the system, the use of thermo-lux fusion strains that express the luxCDABE genes from Xenorhabdus luminescens was evaluated. A total of six recombinant Escherichia coli strains with the promoters from three oxidative-stress responsive genes, i.e. the katG, sodA and pqi-5 genes, fused to either the lux genes from Vibrio fischeri or X. luminescens were characterized and their responses to different chemicals compared. It was found that the basal level bioluminescence (BL) from the thermo-lux fusion strains was always higher while that of the V. fischeri lux strains were always near or below the lower limit of detection of the system. For example, the katG::V. fischeri lux strain, DPD2511, gave no discernible response due to its low level expression while a fusion of the katG promoter with the X. luminescens lux operon was clearly responsive and capable of detecting hydrogen peroxide down to about 1 ppm. The use of the thermo-lux strains found them to be as sensitive as the V. fischeri lux strains while providing a brighter, more stable basal level bioluminescence, making the analysis and monitoring of water-borne toxicity more reliable.

AB - The enhancement of the multi-channel continuous toxicity monitoring system developed previously was studied. To achieve better and more stable results from the system, the use of thermo-lux fusion strains that express the luxCDABE genes from Xenorhabdus luminescens was evaluated. A total of six recombinant Escherichia coli strains with the promoters from three oxidative-stress responsive genes, i.e. the katG, sodA and pqi-5 genes, fused to either the lux genes from Vibrio fischeri or X. luminescens were characterized and their responses to different chemicals compared. It was found that the basal level bioluminescence (BL) from the thermo-lux fusion strains was always higher while that of the V. fischeri lux strains were always near or below the lower limit of detection of the system. For example, the katG::V. fischeri lux strain, DPD2511, gave no discernible response due to its low level expression while a fusion of the katG promoter with the X. luminescens lux operon was clearly responsive and capable of detecting hydrogen peroxide down to about 1 ppm. The use of the thermo-lux strains found them to be as sensitive as the V. fischeri lux strains while providing a brighter, more stable basal level bioluminescence, making the analysis and monitoring of water-borne toxicity more reliable.

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Enhancement of the multi-channel continuous monitoring system through the use of Xenorhabdus luminescens lux fusions

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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