Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement

Seung Kyou You; Young Chul Joo; Dae Hee Kang; Sang Kyu Shin; Jeong Eun Hyeon; Han Min Woo; Youngsoon Um; Chulhwan Park; Sung Ok Han

doi:10.1021/acs.jafc.7b04485

Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement

Seung Kyou You, Young Chul Joo, Dae Hee Kang, Sang Kyu Shin, Jeong Eun Hyeon, Han Min Woo, Youngsoon Um, Chulhwan Park, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wild-type strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.

Original language	English
Pages (from-to)	11029-11035
Number of pages	7
Journal	Journal of agricultural and food chemistry
Volume	65
Issue number	50
DOIs	https://doi.org/10.1021/acs.jafc.7b04485
Publication status	Published - 2017 Dec 20

Bibliographical note

Funding Information:
*Phone: +82-2-3290-3151. Fax: +82-2-3290-3151. E-mail: [email protected]. ORCID Young-Chul Joo: 0000-0001-5169-8734 Han Min Woo: 0000-0002-8797-0477 Youngsoon Um: 0000-0003-1695-1874 Sung Ok Han: 0000-0002-2400-2882 Funding This work was supported by the National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (Grant No. CAP-11-04-KIST). Notes The authors declare no competing financial interest.

Publisher Copyright:
© 2017 American Chemical Society.

Keywords

Saccharomyces cerevisiae, nitrogen optimization
animal feed supplement
cofactor balancing
fatty acids production

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/acs.jafc.7b04485

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title = "Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement",

abstract = "Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wild-type strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.",

keywords = "Saccharomyces cerevisiae, nitrogen optimization, animal feed supplement, cofactor balancing, fatty acids production",

author = "You, {Seung Kyou} and Joo, {Young Chul} and Kang, {Dae Hee} and Shin, {Sang Kyu} and Hyeon, {Jeong Eun} and Woo, {Han Min} and Youngsoon Um and Chulhwan Park and Han, {Sung Ok}",

note = "Funding Information: *Phone: +82-2-3290-3151. Fax: +82-2-3290-3151. E-mail: [email protected]. ORCID Young-Chul Joo: 0000-0001-5169-8734 Han Min Woo: 0000-0002-8797-0477 Youngsoon Um: 0000-0003-1695-1874 Sung Ok Han: 0000-0002-2400-2882 Funding This work was supported by the National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (Grant No. CAP-11-04-KIST). Notes The authors declare no competing financial interest. Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

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AU - You, Seung Kyou

AU - Joo, Young Chul

AU - Kang, Dae Hee

AU - Shin, Sang Kyu

AU - Hyeon, Jeong Eun

AU - Woo, Han Min

AU - Um, Youngsoon

AU - Park, Chulhwan

AU - Han, Sung Ok

N1 - Funding Information: *Phone: +82-2-3290-3151. Fax: +82-2-3290-3151. E-mail: [email protected]. ORCID Young-Chul Joo: 0000-0001-5169-8734 Han Min Woo: 0000-0002-8797-0477 Youngsoon Um: 0000-0003-1695-1874 Sung Ok Han: 0000-0002-2400-2882 Funding This work was supported by the National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (Grant No. CAP-11-04-KIST). Notes The authors declare no competing financial interest. Publisher Copyright: © 2017 American Chemical Society.

PY - 2017/12/20

Y1 - 2017/12/20

N2 - Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wild-type strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.

AB - Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wild-type strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.

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Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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