Enhancing poly(3-hydroxybutyrate) production in halophilic bacteria through improved salt tolerance

Yeonjae Yoo, Dae Young Kwon, Minseo Jeon, Jaehoon Lee, Haeun Kwon, Dongho Lee, Jong Seong Khim, Yoon E. Choi, Jae Jin Kim

Research output: Contribution to journalArticlepeer-review


Polyhydroxyalkanoates (PHA) have emerged as a promising bio-compound in the industrial application due to their potential to replace conventional petroleum-based plastics with sustainable bioplastics. This study focuses on Halomonas sp. YJPS3-3, a halophilic bacterium, and presents a novel approach to enhance PHA production by exploiting its salt tolerance toward PHA biosynthesis. Through gamma irradiation-induced mutants with enhanced salt tolerance from 15% NaCl to 20% NaCl, mutant halo6 showing a significant 11% increase in PHA yield, was achieved. Moreover, the mutants displayed not only higher PHA content but also remarkable cell morphology with elongation. In addition, this research unravels the genetic determinants behind the elevated PHA content and identifies a corresponding shift in fatty acid composition favoring PHA accumulation. This novel mutant obtained from gamma irradiation with enhanced salt tolerance in halophilic bacteria opens up new avenues not only for the bioplastic industry but also for applications in the production of high-value metabolites.

Original languageEnglish
Article number130175
JournalBioresource technology
Publication statusPublished - 2024 Feb

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)


  • Halophiles
  • Halotolerance
  • Mutagenesis
  • Poly(3-hydroxybutyrate)
  • Polyhydroxyalkanoates

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


Dive into the research topics of 'Enhancing poly(3-hydroxybutyrate) production in halophilic bacteria through improved salt tolerance'. Together they form a unique fingerprint.

Cite this