Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide

Na Ma; Seung In Hong; Tingting Zhao; Da Som No; Chong Tai Kim; Yangha Kim; In Hwan Kim

doi:10.1016/j.supflu.2013.12.024

Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide

Na Ma, Seung In Hong, Tingting Zhao, Da Som No, Chong Tai Kim, Yangha Kim, In Hwan Kim

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

This study focused on the use of pressurized CO₂ as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO ₂, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO₂, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.

Original language	English
Pages (from-to)	28-33
Number of pages	6
Journal	Journal of Supercritical Fluids
Volume	87
DOIs	https://doi.org/10.1016/j.supflu.2013.12.024
Publication status	Published - 2014 Mar

Bibliographical note

Funding Information:
This study was supported by the Food High Pressure Technology Development Project, Korea Food Research Institute, Korea .

Keywords

Docosahexaenoic acid (DHA)
Ethanol
Lipozyme RM IM (Rhizomucor miehei)
Near-supercritical carbon dioxide
Tuna oil fatty acids

ASJC Scopus subject areas

General Chemical Engineering
Condensed Matter Physics
Physical and Theoretical Chemistry

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10.1016/j.supflu.2013.12.024

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title = "Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide",

abstract = "This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO 2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.",

keywords = "Docosahexaenoic acid (DHA), Ethanol, Lipozyme RM IM (Rhizomucor miehei), Near-supercritical carbon dioxide, Tuna oil fatty acids",

author = "Na Ma and Hong, {Seung In} and Tingting Zhao and No, {Da Som} and Kim, {Chong Tai} and Yangha Kim and Kim, {In Hwan}",

note = "Funding Information: This study was supported by the Food High Pressure Technology Development Project, Korea Food Research Institute, Korea .",

year = "2014",

month = mar,

doi = "10.1016/j.supflu.2013.12.024",

language = "English",

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T1 - Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide

AU - Ma, Na

AU - Hong, Seung In

AU - Zhao, Tingting

AU - No, Da Som

AU - Kim, Chong Tai

AU - Kim, Yangha

AU - Kim, In Hwan

N1 - Funding Information: This study was supported by the Food High Pressure Technology Development Project, Korea Food Research Institute, Korea .

PY - 2014/3

Y1 - 2014/3

N2 - This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO 2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.

AB - This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO 2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.

KW - Docosahexaenoic acid (DHA)

KW - Ethanol

KW - Lipozyme RM IM (Rhizomucor miehei)

KW - Near-supercritical carbon dioxide

KW - Tuna oil fatty acids

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Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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