Abstract
Erucic acid enrichment from crambe oil was accomplished in a recirculated packed bed (RPBR) reactor via ethanolysis using Novozym 435 lipase from Candida antarctica as a biocatalyst. The content of erucic acid of the crambe oil used was 59.8 mol% The erucic acid was located predominantly in the sn-1,3 position of the triacylglycerol of the crambe oil. Novozym 435 used in this study has selectivity toward fatty acids in sn-1,3 position of triglyceride in excess ethanol. The effects of reaction temperature, molar ratio, and residence time of the substrate in RPBR on the enrichment of erucic acid as a function of reaction time were studied. The optimal temperature, molar ratio (crambe oil to ethanol), and residence time of the substrate in RPBR were 45 °C, 1:60, and 4 min, respectively.
Original language | English |
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Pages (from-to) | 6-10 |
Number of pages | 5 |
Journal | Journal of Molecular Catalysis B: Enzymatic |
Volume | 87 |
DOIs | |
Publication status | Published - 2013 Mar |
Keywords
- Candida antartica
- Crambe oil
- Erucic acid
- Ethanolysis
- Recirculated packed bed reactor (RPBR)
ASJC Scopus subject areas
- Catalysis
- Bioengineering
- Biochemistry
- Process Chemistry and Technology