Abstract
A commercial phospholipase A1 (Lecltase® Ultra) was Immobilized by physical adsorption on Duolite®and then used to mediate the incorporation of omega-3 fatty acids into lecithin. Adsorption isotherms showed that 12 h of contact were sufficient to deposit most of the enzyme onto the carrier. A pH of 7 and 50°C were the best conditions for adsorption. Reaction mixtures consisting of lecithin and a saponified fish oil concentrate (78.4 mol % EPA+DPA+DHA) were prepared at molar ratios ranging from 1:2 to 1:10. Typically 2 g of total substrates and 200 mg of enzyme preparation were employed in batch reactor trials. The fastest reaction rates were observed when a substrate mole ratio of 1:8 (lecithin:total fatty acids) was employed. Use of the enzyme preparation dried at pH 8 and reaction temperatures of 50 and 60°C produced the greatest extent of incorporation of the indicated n-3 fatty acids into the phospholipid after 24h of reaction.
Original language | English |
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Pages (from-to) | 368-374 |
Number of pages | 7 |
Journal | Grasas y Aceites |
Volume | 59 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2008 |
Keywords
- Fish oil
- Interesterification
- Omega-3
- Phospholipids
ASJC Scopus subject areas
- Food Science
- Organic Chemistry