Enzymatic degradation of lignocellulosic biomass by continuous process using laccase and cellulases with the aid of scaffoldin for ethanol production

Jeong Eun Hyeon, Seung Kyou You, Dae Hee Kang, Sun Hwa Ryu, Myungkil Kim, Sung Suk Lee, Sung Ok Han

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Biological processes for the degradation of intractable materials are still not considered to be practical due to the slow rates of enzymatic degradation. Cellulosomes are complexed enzyme systems with great degradative potential and one of the strategies for overcoming this problem. In this study, the laccase CueO from Escherichia coli was fused to the dockerin domain of a cellulosome system and further assembled with the scaffoldin miniCbpA, forming a laccase-miniCbpA complex. Compared to the individual subunits, laccase-miniCbpA complex caused a noticeable 2.1-fold increase in enzyme activity levels and enhanced degradation of various synthetic dyes, showing an increase of approximately 1.6-fold. Also, pretreated barley straw by laccase complexes was efficiently converted to bioethanol using a cellulase producing Saccharomyces cerevisiae strain. The laccase complexes caused a 2.6-fold increase in the amount of reduced sugar with an insoluble substrate in conditions with an identical amount of enzymes. The cellulolytic yeast with the aid of laccase complexes produced 2.34 g/L ethanol after 72 h, indicating an increase of approximately 2.1-fold compared to fermentation without the laccase complexes. This demonstrates the feasibility of developing an efficient laccase complex based on the cellulosome and this strategy may be used to degrade recalcitrant materials.

Original languageEnglish
Pages (from-to)1266-1273
Number of pages8
JournalProcess Biochemistry
Issue number8
Publication statusPublished - 2014 Aug

Bibliographical note

Funding Information:
This research was supported in part by a research grant provided by the Korea Forest Research Institute (KFRI) and the New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korean government Ministry of Knowledge Economy (No. 20113010090040 ).


  • Bioethanol
  • Cellulosome
  • Decolorization
  • Laccase
  • Laccase complex
  • Lignocellulose pretreatment

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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