TY - GEN
T1 - Enzymatic glucose biosensor based on porous ZnO/Au electrodes
AU - You, Xueqiu
AU - Park, Jungil
AU - Jang, Yunseok
AU - Kim, Soo Won
AU - Pak, James Jungho
AU - Min, Nam Ki
PY - 2010
Y1 - 2010
N2 - This paper describes a glucose biosensor based on glucose oxidase (GOx) immobilized on porous ZnO/Au electrodes. The ZnO porous electrodes were fabricated by electrochemical deposition of zinc oxide on patterned Au electrodes using polystyrene (PS) spheres as templates. Uniform pore size and highly ordered ZnO pore arrangement were observed from SEM images. X-ray diffraction (XRD) patterns revealed a single crystalline nature of the porous ZnO. This porous structure provides high enzyme loading capacity and long-term stability. In a pH 7.4 phosphate buffer solution, the positively charged high isoelectric point (IEP) ZnO pores enhance the adsorption of negatively charged low IEP GOx through electrostatic attractive force. Once GOx molecules are immobilized within the ZnO pores, the bottleneck structure resulting from the connected pores hinders leaching of GOx from the pores. The resulting enzymatic biosensor showed a linear detection range from 1mM to 18mM, and sensitivity of 10.89μA/ (mM·cm2) with good selectivity and long-term stability.
AB - This paper describes a glucose biosensor based on glucose oxidase (GOx) immobilized on porous ZnO/Au electrodes. The ZnO porous electrodes were fabricated by electrochemical deposition of zinc oxide on patterned Au electrodes using polystyrene (PS) spheres as templates. Uniform pore size and highly ordered ZnO pore arrangement were observed from SEM images. X-ray diffraction (XRD) patterns revealed a single crystalline nature of the porous ZnO. This porous structure provides high enzyme loading capacity and long-term stability. In a pH 7.4 phosphate buffer solution, the positively charged high isoelectric point (IEP) ZnO pores enhance the adsorption of negatively charged low IEP GOx through electrostatic attractive force. Once GOx molecules are immobilized within the ZnO pores, the bottleneck structure resulting from the connected pores hinders leaching of GOx from the pores. The resulting enzymatic biosensor showed a linear detection range from 1mM to 18mM, and sensitivity of 10.89μA/ (mM·cm2) with good selectivity and long-term stability.
KW - Enzyme immobilization
KW - Glucose biosensor
KW - Glucose oxidase
KW - ZnO porous structure
UR - http://www.scopus.com/inward/record.url?scp=79956026671&partnerID=8YFLogxK
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U2 - 10.1109/NANOMED.2010.5749805
DO - 10.1109/NANOMED.2010.5749805
M3 - Conference contribution
AN - SCOPUS:79956026671
SN - 9781612841533
T3 - 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
SP - 56
EP - 59
BT - 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
T2 - 4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Y2 - 5 December 2010 through 9 December 2010
ER -