Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

Seung Phill Choi, Minh Thu Nguyen, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

293 Citations (Scopus)


The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.

Original languageEnglish
Pages (from-to)5330-5336
Number of pages7
JournalBioresource technology
Issue number14
Publication statusPublished - 2010 Jul
Externally publishedYes


  • Chlamydomonas reinhardtii
  • Enzymatic pretreatment
  • Ethanol
  • Microalgae

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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