Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

Seung Phill Choi; Minh Thu Nguyen; Sang Jun Sim

doi:10.1016/j.biortech.2010.02.026

Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

Seung Phill Choi, Minh Thu Nguyen, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

293 Citations (Scopus)

Abstract

The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.

Original language	English
Pages (from-to)	5330-5336
Number of pages	7
Journal	Bioresource technology
Volume	101
Issue number	14
DOIs	https://doi.org/10.1016/j.biortech.2010.02.026
Publication status	Published - 2010 Jul
Externally published	Yes

Keywords

Chlamydomonas reinhardtii
Enzymatic pretreatment
Ethanol
Microalgae

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2010.02.026

Cite this

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title = "Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production",

abstract = "The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.",

keywords = "Chlamydomonas reinhardtii, Enzymatic pretreatment, Ethanol, Microalgae",

author = "Choi, {Seung Phill} and Nguyen, {Minh Thu} and Sim, {Sang Jun}",

note = "Funding Information: This research was supported by a grant (DG2-201) from the Carbon Dioxide Reduction and Sequestration Research Center, one of the 21st Century Frontier Programs that was funded by the Ministry of Science and Technology of the Korean Government.",

year = "2010",

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doi = "10.1016/j.biortech.2010.02.026",

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AU - Choi, Seung Phill

AU - Nguyen, Minh Thu

AU - Sim, Sang Jun

N1 - Funding Information: This research was supported by a grant (DG2-201) from the Carbon Dioxide Reduction and Sequestration Research Center, one of the 21st Century Frontier Programs that was funded by the Ministry of Science and Technology of the Korean Government.

PY - 2010/7

Y1 - 2010/7

N2 - The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.

AB - The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.

KW - Chlamydomonas reinhardtii

KW - Enzymatic pretreatment

KW - Ethanol

KW - Microalgae

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JO - Bioresource Technology

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ER -

Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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