Enzymatic production of sugar from fungi and fungi-infected lignocellulosic biomass by a new cellulosomal enzyme harboring N-acetyl-β-D-glucosaminidase activity

Myeong Eun Lee, Sang Kyu Shin, Jeong Joo Oh, Dong Hyeok Hwang, Young Jin Ko, Jeong Eun Hyeon, Sung Ok Han

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Cellulosomes are scaffold proteins displaying enzymes on the cell wall to efficiently obtain nutrient sources. CcGlcNAcase is a novel cellulosomal component. Based on sequence analysis, CcGlcNAcase was predicted to be a chitinolytic enzyme based on high homology with the discoidin domain-containing protein and chitobiase/ β-hexosaminidase C terminal domain. CcGlcNAcase expression was notably increased when chitin was present. CcGlcNAcase produced N-acetyl-D-glucosamine from various lengths of N-acetyl-D-glucosamine. CcGlcNAcase bound to chitin (89%) and fungi (54.10%), whereas CcGlcNAcase exhibited a low binding ability to cellulose and xylan. CcGlcNAcase hydrolyzed fungi, yielding maximum 3.90 g/L N-acetyl-D-glucosamine. CcGlcNAcase enhanced cellulase toward fungi-infected lignocellulosic biomass, yielding 18 mg/L glucose (1.32-fold) and 1.72-fold increased total reducing sugar levels, whereas cellulase alone produced 13 mg/L glucose. Taken together, CcGlcNAcase can be utilized to enhance the degradation of fungi-infected lignocellulosic biomass and exhibits potential applications in the wood and sugar industry.

    Original languageEnglish
    Article number124242
    JournalBioresource technology
    Volume319
    DOIs
    Publication statusPublished - 2021 Jan

    Bibliographical note

    Funding Information:
    This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. 2018R1A2B2003704 ) and Korea University Grant.

    Publisher Copyright:
    © 2020 Elsevier Ltd

    Keywords

    • Cellulosome
    • Chitin
    • Clostridium cellulovorans
    • Fungi degradation
    • N-acetyl-β-D-glucosaminidase

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Renewable Energy, Sustainability and the Environment
    • Waste Management and Disposal

    Fingerprint

    Dive into the research topics of 'Enzymatic production of sugar from fungi and fungi-infected lignocellulosic biomass by a new cellulosomal enzyme harboring N-acetyl-β-D-glucosaminidase activity'. Together they form a unique fingerprint.

    Cite this