Abstract
This study aimed to enzymatically prepare structured monogalactosyldiacylglycerols (MGDGs) with different hydrophile-lipophile balance (HLB) values for use as emulsifiers. Acidolysis of Perilla frutescens-derived MGDGs with capric acid (10:0) was conducted to obtain structured MGDGs containing 10:0. Lewatit VP OC 1600-immobilized Rhizomucor miehei lipase was used as the biocatalyst. Structured MGDGs (HLB value = 2.95–7.17) containing 13.0–70.6 mol% 10:0 were obtained from P. frutescens MGDGs (HLB value = 1.93). A quadratic regression equation (R2 = 0.920) to predict the 10:0 content of the structured MGDGs under the given conditions was established using response surface methodology. Using a linear regression equation (R2 = 0.999) to predict the HLB value by 10:0 content, structured MGDGs containing 27.1–54.6 mol% 10:0 were predicted to have an HLB value of 4–6, indicating their potential applicability as hydrophobic emulsifiers. Structured MGDGs with a purity of ∼ 43% w/w were obtained from the reaction products using silica column chromatography.
Original language | English |
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Article number | 132705 |
Journal | Food Chemistry |
Volume | 385 |
DOIs | |
Publication status | Published - 2022 Aug 15 |
Bibliographical note
Funding Information:This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) [No. 2019R1F1A1053604].
Publisher Copyright:
© 2022 Elsevier Ltd
Keywords
- Capric acid
- Emulsifier
- Hydrophile-lipophile balance
- Immobilization
- Rhizomucor miehei lipase
- Structured monogalactosyldiacylglycerol
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science