Enzymatic structural modification of monogalactosyldiacylglycerols for potential modulation of hydrophile-lipophile balance

Seoye Roh, Sohee Lee, In Hwan Kim, Byung Hee Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This study aimed to enzymatically prepare structured monogalactosyldiacylglycerols (MGDGs) with different hydrophile-lipophile balance (HLB) values for use as emulsifiers. Acidolysis of Perilla frutescens-derived MGDGs with capric acid (10:0) was conducted to obtain structured MGDGs containing 10:0. Lewatit VP OC 1600-immobilized Rhizomucor miehei lipase was used as the biocatalyst. Structured MGDGs (HLB value = 2.95–7.17) containing 13.0–70.6 mol% 10:0 were obtained from P. frutescens MGDGs (HLB value = 1.93). A quadratic regression equation (R2 = 0.920) to predict the 10:0 content of the structured MGDGs under the given conditions was established using response surface methodology. Using a linear regression equation (R2 = 0.999) to predict the HLB value by 10:0 content, structured MGDGs containing 27.1–54.6 mol% 10:0 were predicted to have an HLB value of 4–6, indicating their potential applicability as hydrophobic emulsifiers. Structured MGDGs with a purity of ∼ 43% w/w were obtained from the reaction products using silica column chromatography.

Original languageEnglish
Article number132705
JournalFood Chemistry
Volume385
DOIs
Publication statusPublished - 2022 Aug 15

Bibliographical note

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

  • Capric acid
  • Emulsifier
  • Hydrophile-lipophile balance
  • Immobilization
  • Rhizomucor miehei lipase
  • Structured monogalactosyldiacylglycerol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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