Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents

Kyung Min Yeon; Jisung You; Manab Deb Adhikari; Sung Gil Hong; Inseon Lee; Han Sol Kim; Li Na Kim; Jahyun Nam; Seok Joon Kwon; Moon Il Kim; Warayuth Sajomsang; Jonathan S. Dordick; Jungbae Kim

doi:10.1021/acs.biomac.9b00152

Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents

Kyung Min Yeon, Jisung You, Manab Deb Adhikari, Sung Gil Hong, Inseon Lee, Han Sol Kim, Li Na Kim, Jahyun Nam, Seok Joon Kwon, Moon Il Kim, Warayuth Sajomsang, Jonathan S. Dordick, Jungbae Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Highly effective and minimally toxic antimicrobial agents have been prepared by immobilizing glucose oxidase (GOx) onto biocompatible chitosan nanoparticles (CS-NPs). CS-NPs were prepared via ionotropic gelation and used for the immobilization of GOx via approaches of covalent attachment (CA), enzyme coating (EC), enzyme precipitate coating (EPC), and magnetic nanoparticle-incorporated EPC (Mag-EPC). EPC represents an approach consisting of enzyme covalent attachment, precipitation, and cross-linking, with CA and EC being control samples while Mag-EPC was prepared by mixing magnetic nanoparticles (Mag) with enzymes during the preparation of EPC. The GOx activities of CA, EC, EPC, and Mag-EPC were 8.57, 17.7, 219, and 247 units/mg CS-NPs, respectively, representing 26 and 12 times higher activity of EPC than those of CA and EC, respectively. EPC improved the activity and stability of GOx and led to good dispersion of CS-NPs, while Mag-EPC enabled facile magnetic separation. To demonstrate the expandability of the EPC approach to other enzymes, bovine carbonic anhydrase was also employed to prepare EPC and Mag-EPC samples for their characterizations. In the presence of glucose, EPC of GOx generated H₂O₂ in situ, which effectively inhibited the proliferation of Staphylococcus aureus in both suspended cultures and biofilms, thereby demonstrating the potential of EPC-GOx as environmentally friendly and highly effective antimicrobial materials.

Original language	English
Pages (from-to)	2477-2485
Number of pages	9
Journal	Biomacromolecules
Volume	20
Issue number	7
DOIs	https://doi.org/10.1021/acs.biomac.9b00152
Publication status	Published - 2019 Jul 8

Bibliographical note

Funding Information:
This work was supported by the Global Research Laboratory Program (2014K1A1A2043032) and the Nano Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT. This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (no. 20182010600430).

Funding Information:
This work was supported by the Global Research Laboratory Program (2014K1A1A2043032) and the Nano Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT. This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (no. 20182010600430).

Publisher Copyright:
© 2019 American Chemical Society.

ASJC Scopus subject areas

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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title = "Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents",

abstract = "Highly effective and minimally toxic antimicrobial agents have been prepared by immobilizing glucose oxidase (GOx) onto biocompatible chitosan nanoparticles (CS-NPs). CS-NPs were prepared via ionotropic gelation and used for the immobilization of GOx via approaches of covalent attachment (CA), enzyme coating (EC), enzyme precipitate coating (EPC), and magnetic nanoparticle-incorporated EPC (Mag-EPC). EPC represents an approach consisting of enzyme covalent attachment, precipitation, and cross-linking, with CA and EC being control samples while Mag-EPC was prepared by mixing magnetic nanoparticles (Mag) with enzymes during the preparation of EPC. The GOx activities of CA, EC, EPC, and Mag-EPC were 8.57, 17.7, 219, and 247 units/mg CS-NPs, respectively, representing 26 and 12 times higher activity of EPC than those of CA and EC, respectively. EPC improved the activity and stability of GOx and led to good dispersion of CS-NPs, while Mag-EPC enabled facile magnetic separation. To demonstrate the expandability of the EPC approach to other enzymes, bovine carbonic anhydrase was also employed to prepare EPC and Mag-EPC samples for their characterizations. In the presence of glucose, EPC of GOx generated H2O2 in situ, which effectively inhibited the proliferation of Staphylococcus aureus in both suspended cultures and biofilms, thereby demonstrating the potential of EPC-GOx as environmentally friendly and highly effective antimicrobial materials.",

author = "Yeon, {Kyung Min} and Jisung You and Adhikari, {Manab Deb} and Hong, {Sung Gil} and Inseon Lee and Kim, {Han Sol} and Kim, {Li Na} and Jahyun Nam and Kwon, {Seok Joon} and Kim, {Moon Il} and Warayuth Sajomsang and Dordick, {Jonathan S.} and Jungbae Kim",

note = "Funding Information: This work was supported by the Global Research Laboratory Program (2014K1A1A2043032) and the Nano Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT. This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (no. 20182010600430). Funding Information: This work was supported by the Global Research Laboratory Program (2014K1A1A2043032) and the Nano Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT. This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (no. 20182010600430). Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

year = "2019",

month = jul,

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doi = "10.1021/acs.biomac.9b00152",

language = "English",

volume = "20",

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journal = "Biomacromolecules",

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T1 - Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents

AU - Yeon, Kyung Min

AU - You, Jisung

AU - Adhikari, Manab Deb

AU - Hong, Sung Gil

AU - Lee, Inseon

AU - Kim, Han Sol

AU - Kim, Li Na

AU - Nam, Jahyun

AU - Kwon, Seok Joon

AU - Kim, Moon Il

AU - Sajomsang, Warayuth

AU - Dordick, Jonathan S.

AU - Kim, Jungbae

N1 - Funding Information: This work was supported by the Global Research Laboratory Program (2014K1A1A2043032) and the Nano Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT. This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (no. 20182010600430). Funding Information: This work was supported by the Global Research Laboratory Program (2014K1A1A2043032) and the Nano Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT. This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (no. 20182010600430). Publisher Copyright: © 2019 American Chemical Society.

PY - 2019/7/8

Y1 - 2019/7/8

N2 - Highly effective and minimally toxic antimicrobial agents have been prepared by immobilizing glucose oxidase (GOx) onto biocompatible chitosan nanoparticles (CS-NPs). CS-NPs were prepared via ionotropic gelation and used for the immobilization of GOx via approaches of covalent attachment (CA), enzyme coating (EC), enzyme precipitate coating (EPC), and magnetic nanoparticle-incorporated EPC (Mag-EPC). EPC represents an approach consisting of enzyme covalent attachment, precipitation, and cross-linking, with CA and EC being control samples while Mag-EPC was prepared by mixing magnetic nanoparticles (Mag) with enzymes during the preparation of EPC. The GOx activities of CA, EC, EPC, and Mag-EPC were 8.57, 17.7, 219, and 247 units/mg CS-NPs, respectively, representing 26 and 12 times higher activity of EPC than those of CA and EC, respectively. EPC improved the activity and stability of GOx and led to good dispersion of CS-NPs, while Mag-EPC enabled facile magnetic separation. To demonstrate the expandability of the EPC approach to other enzymes, bovine carbonic anhydrase was also employed to prepare EPC and Mag-EPC samples for their characterizations. In the presence of glucose, EPC of GOx generated H2O2 in situ, which effectively inhibited the proliferation of Staphylococcus aureus in both suspended cultures and biofilms, thereby demonstrating the potential of EPC-GOx as environmentally friendly and highly effective antimicrobial materials.

AB - Highly effective and minimally toxic antimicrobial agents have been prepared by immobilizing glucose oxidase (GOx) onto biocompatible chitosan nanoparticles (CS-NPs). CS-NPs were prepared via ionotropic gelation and used for the immobilization of GOx via approaches of covalent attachment (CA), enzyme coating (EC), enzyme precipitate coating (EPC), and magnetic nanoparticle-incorporated EPC (Mag-EPC). EPC represents an approach consisting of enzyme covalent attachment, precipitation, and cross-linking, with CA and EC being control samples while Mag-EPC was prepared by mixing magnetic nanoparticles (Mag) with enzymes during the preparation of EPC. The GOx activities of CA, EC, EPC, and Mag-EPC were 8.57, 17.7, 219, and 247 units/mg CS-NPs, respectively, representing 26 and 12 times higher activity of EPC than those of CA and EC, respectively. EPC improved the activity and stability of GOx and led to good dispersion of CS-NPs, while Mag-EPC enabled facile magnetic separation. To demonstrate the expandability of the EPC approach to other enzymes, bovine carbonic anhydrase was also employed to prepare EPC and Mag-EPC samples for their characterizations. In the presence of glucose, EPC of GOx generated H2O2 in situ, which effectively inhibited the proliferation of Staphylococcus aureus in both suspended cultures and biofilms, thereby demonstrating the potential of EPC-GOx as environmentally friendly and highly effective antimicrobial materials.

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Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents

Abstract

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