Enzyme precipitate coating of pyranose oxidase on carbon nanotubes and their electrochemical applications

Jae Hyun Kim, Sung Gil Hong, Youngho Wee, Shuozhen Hu, Yongchai Kwon, Su Ha, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


Pyranose oxidase (POx), which doesn't have electrically non-conductive glycosylation moiety, was immobilized on carbon nanotubes (CNTs) via three different preparation methods: covalent attachment (CA), enzyme coating (EC) and enzyme precipitate coating (EPC). CA, EC and EPC of POx on CNTs were used to fabricate enzymatic electrodes for enzyme-based biosensors and biofuel cells. Improved enzyme loading of EPC resulted in 6.5 and 4.5 times higher activity per weight of CNTs than those of CA and EC, respectively. After 34 days at room temperature, EPC retained 65% of initial activity, while CA and EC maintained 9.2% and 26% of their initial activities, respectively. These results indicate that precipitation and crosslinking steps of EPC have an important role in maintaining enzyme activity. To demonstrate the feasibility of POx-based biosensors and biofuel cells, the enzyme electrodes were prepared using CA, EC, and EPC samples. In the case of biosensor, the sensitivities of the CA, EC, and EPC electrodes without BQ were measured to be 0.27, 0.76 and 3.7 mA/M/cm2, while CA, EC and EPC electrode with BQ showed 25, 25, and 60 mA/M/cm2 of sensitivities, respectively. The maximum power densities of biofuel cells using CA, EC and EPC electrodes without BQ were 41, 47 and 53 µW/cm2, while CA, EC and EPC electrodes with BQ showed 260, 330 and 500 µW/cm2, respectively. The POx immobilization and stabilization via the EPC approach can lead us to develop continuous glucose monitoring biosensors and high performing biofuel cells.

Original languageEnglish
Pages (from-to)365-372
Number of pages8
JournalBiosensors and Bioelectronics
Publication statusPublished - 2017 Jan 15

Bibliographical note

Funding Information:
This research was supported by Global Research Laboratory Program ( 2014K1A1A2043032 ), Nano Material Technology Development Program ( 2014M3A7B4052193 ), and ‘2016, University-Institute cooperation program’ through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning . This research was also supported by the Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) and the Ministry of Trade, Industry & Energy ( MOTIE ) of the Republic of Korea (No. 20142020200980 ).

Publisher Copyright:
© 2016 Elsevier B.V.


  • Carbon nanotubes
  • Enzyme based biofuel cells
  • Enzyme precipitate coatings
  • Glucose biosensors
  • Pyranose oxidase

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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