Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications

Mike Fischback, Ki Young Kwon, Inseon Lee, Su Jeong Shin, Hyun Gyu Park, Byoung Chan Kim, Yongchai Kwon, Hee Tae Jung, Jungbae Kim, Su Ha

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Enzymatic biofuel cells (BFC) have a great potential as a small power source, but their practical applications are being hampered by short lifetime and low power density. This study describes the direct immobilization of glucose oxidase (GOx) onto the carbon paper in the form of highly stable and active enzyme precipitation coatings (EPCs), which can improve the lifetime and power density of BFCs. EPCs were fabricated directly onto the carbon paper via a three-step process: covalent attachment (CA), enzyme precipitation, and chemical crosslinking. GOx-immobilized carbon papers via the CA and EPC approaches were used as an enzyme anode and their electrochemical activities were tested under the BFC-operating mode. The BFCs with CA and EPC enzyme anodes produced the maximum power densities of 50 and 250μW/cm 2, respectively. The BFC with the EPC enzyme anode showed a stable current density output of >700μA/cm 2 at 0.18V under continuous operation for over 45h. When a maple syrup was used as a fuel under ambient conditions, it also produced a stable current density of >10μA/cm 2 at 0.18V for over 25h. It is anticipated that the direct immobilization of EPC on hierarchical-structured electrodes with a large surface area would further improve the power density of BFCs that can make their applications more feasible.

Original languageEnglish
Pages (from-to)318-324
Number of pages7
JournalBiotechnology and Bioengineering
Issue number2
Publication statusPublished - 2012 Feb


  • Biofuel cell
  • Carbon paper
  • Enzyme precipitation and coating
  • Glucose oxidase
  • Maple syrup

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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