TY - JOUR
T1 - Eotaxin mRNA expression in bronchial mucosa of patients with asthma
AU - Kwang He In, He In
AU - Jae Yun Cho, Yun Cho
AU - Sae Yong Kang, Yong Kang
AU - Sang Youb Lee, Youb Lee
AU - Jae Jeong Shim, Jeong Shim
AU - Kyung Ho Kang, Ho Kang
AU - Se Hwa Yoo, Hwa Yoo
AU - Young Soon Na, Soon Na
AU - Han Gyum Kim, Gyum Kim
PY - 1998
Y1 - 1998
N2 - Background: Asthma is a chronic inflammatory disease of the airways characterized by a marked infiltration of eosinophils in the bronchial mucosa. Asthmatic bronchial mucosa produces many factors described as being chemotactic for inflammatory cells. IL-5, RANTES, and MCP-1 alpha are the chemotactic factors for eosinophils, but their roles are controversial. Recently eotaxin that is a potent eosinophil chemoattractant cytokine was detected in a guinea-pig model of allergic airway inflammation, and human eotaxin was cloned. Eotaxin is a specific chemoattractant for eosinophils, but its role in asthma is not confirmed. We examined the in vivo expression of eotaxin in bronchi of asthmatic patients. Methods: 11 asthmatics and 2 normal controls were enrolled. All subjects were underwent bronchoscopy with bronchial biopsies in 2nd or 3rd carina. RNA extraction from biopsy samples was done by acid-guanidium method. Semi-quantitative RT-PCR was done for evaluation of eotaxin mRNA expression. The extent of eosinophil infiltration was evaluated by counting the eosinophils in submucosa in HPF of microscope. Results: Eotaxin mRNA expressed in symptomatic, uncontrolled asthma. Steroid inhibited expression of eotaxin mRNA in asthma. Expression of eotaxin mRNA correlated with eosinophil infiltration in bronchial tissues. Conclusion: Expression of eotaxin mRNA increases in uncontrolled asthma and eotaxin is involved in the recruitment of eosinophils.
AB - Background: Asthma is a chronic inflammatory disease of the airways characterized by a marked infiltration of eosinophils in the bronchial mucosa. Asthmatic bronchial mucosa produces many factors described as being chemotactic for inflammatory cells. IL-5, RANTES, and MCP-1 alpha are the chemotactic factors for eosinophils, but their roles are controversial. Recently eotaxin that is a potent eosinophil chemoattractant cytokine was detected in a guinea-pig model of allergic airway inflammation, and human eotaxin was cloned. Eotaxin is a specific chemoattractant for eosinophils, but its role in asthma is not confirmed. We examined the in vivo expression of eotaxin in bronchi of asthmatic patients. Methods: 11 asthmatics and 2 normal controls were enrolled. All subjects were underwent bronchoscopy with bronchial biopsies in 2nd or 3rd carina. RNA extraction from biopsy samples was done by acid-guanidium method. Semi-quantitative RT-PCR was done for evaluation of eotaxin mRNA expression. The extent of eosinophil infiltration was evaluated by counting the eosinophils in submucosa in HPF of microscope. Results: Eotaxin mRNA expressed in symptomatic, uncontrolled asthma. Steroid inhibited expression of eotaxin mRNA in asthma. Expression of eotaxin mRNA correlated with eosinophil infiltration in bronchial tissues. Conclusion: Expression of eotaxin mRNA increases in uncontrolled asthma and eotaxin is involved in the recruitment of eosinophils.
KW - Asthma
KW - Bronchial mucosa
KW - Eotaxin
UR - http://www.scopus.com/inward/record.url?scp=0031760575&partnerID=8YFLogxK
U2 - 10.4046/trd.1998.45.4.697
DO - 10.4046/trd.1998.45.4.697
M3 - Article
AN - SCOPUS:0031760575
SN - 0378-0066
VL - 45
SP - 697
EP - 704
JO - Tuberculosis and Respiratory Diseases
JF - Tuberculosis and Respiratory Diseases
IS - 4
ER -