(-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation

Mi Ran Byun, Mi Kyung Sung, A. Rum Kim, Cham Han Lee, Eun Jung Jang, Mi Gyeong Jeong, Minsoo Noh, Eun Sook Hwang, Jeong Ho Hong

    Research output: Contribution to journalArticlepeer-review

    49 Citations (Scopus)

    Abstract

    Osteoporosis is a degenerative bone disease characterized by low bone mass and is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. It is known that the bioactive compounds present in green tea increase osteogenic activity and decrease the risk of fracture by improving bone mineral density. However, the detailed mechanism underlying these beneficial effects has yet to be elucidated. In this study, we investigated the osteogenic effect of (-)-epicatechin gallate (ECG), a major bioactive compound found in green tea. We found that ECG effectively stimulates osteoblast differentiation, indicated by the increased expression of osteoblastic marker genes. Up-regulation of osteoblast marker genes is mediated by increased expression and interaction of the transcriptional coactivator with PDZ-binding motif (TAZ) and Runt-related transcription factor 2 (RUNX2). ECG facilitates nuclear localization ofTAZthrough PP1A. PP1A is essential for osteoblast differentiation because inhibition of PP1A activity was shown to suppress ECG-mediated osteogenic differentiation. Taken together, the results showed that ECG stimulates osteoblast differentiation through the activation of TAZ and RUNX2, revealing a novel mechanism for green tea-stimulated osteoblast differentiation.

    Original languageEnglish
    Pages (from-to)9926-9935
    Number of pages10
    JournalJournal of Biological Chemistry
    Volume289
    Issue number14
    DOIs
    Publication statusPublished - 2014 Apr 4

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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