ERα regulates chromosome alignment and spindle dynamics during mitosis

Xianghua Zhang; Hweon Park; Sung Sik Han; Jung Woo Kim; Chang Young Jang

doi:10.1016/j.bbrc.2014.12.062

ERα regulates chromosome alignment and spindle dynamics during mitosis

Xianghua Zhang, Hweon Park, Sung Sik Han, Jung Woo Kim, Chang Young Jang

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Estrogen receptors are activated by the hormone estrogen and they control cell growth by altering gene expression as a transcription factor. So far two estrogen receptors have been found: ERα and ERβ. Estrogen receptors are also implicated in the development and progression of breast cancer. Here, we found that ERα localized on the spindle and spindle poles at the metaphase during mitosis. Depletion of ERα generated unaligned chromosomes in metaphase cells and lagging chromosomes in anaphase cells in a transcription-independent manner. Furthermore, the levels of β-tubulin and γ-tubulin were reduced in ERα-depleted cells. Consistent with this, polymerization of microtubules in ERα-depleted cells and turnover rate of α/β-tubulin were decreased than in control cells. We suggest that ERα regulates chromosome alignment and spindle dynamics by stabilizing microtubules during mitosis.

Original language	English
Pages (from-to)	919-925
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	456
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2014.12.062
Publication status	Published - 2015 Jan 24

Bibliographical note

Funding Information:
This work was supported by the SRC Research Center for Women’s Diseases of Sookmyung Women’s University (2011).

Publisher Copyright:
© 2014 Elsevier Inc. All rights reserved.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

Chromosome movement
ERα
Mitotic spindle
Spindle dynamics

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2014.12.062

Cite this

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title = "ERα regulates chromosome alignment and spindle dynamics during mitosis",

abstract = "Estrogen receptors are activated by the hormone estrogen and they control cell growth by altering gene expression as a transcription factor. So far two estrogen receptors have been found: ERα and ERβ. Estrogen receptors are also implicated in the development and progression of breast cancer. Here, we found that ERα localized on the spindle and spindle poles at the metaphase during mitosis. Depletion of ERα generated unaligned chromosomes in metaphase cells and lagging chromosomes in anaphase cells in a transcription-independent manner. Furthermore, the levels of β-tubulin and γ-tubulin were reduced in ERα-depleted cells. Consistent with this, polymerization of microtubules in ERα-depleted cells and turnover rate of α/β-tubulin were decreased than in control cells. We suggest that ERα regulates chromosome alignment and spindle dynamics by stabilizing microtubules during mitosis.",

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author = "Xianghua Zhang and Hweon Park and Han, {Sung Sik} and Kim, {Jung Woo} and Jang, {Chang Young}",

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doi = "10.1016/j.bbrc.2014.12.062",

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AU - Zhang, Xianghua

AU - Park, Hweon

AU - Han, Sung Sik

AU - Kim, Jung Woo

AU - Jang, Chang Young

N1 - Funding Information: This work was supported by the SRC Research Center for Women’s Diseases of Sookmyung Women’s University (2011). Publisher Copyright: © 2014 Elsevier Inc. All rights reserved. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 2015/1/24

Y1 - 2015/1/24

N2 - Estrogen receptors are activated by the hormone estrogen and they control cell growth by altering gene expression as a transcription factor. So far two estrogen receptors have been found: ERα and ERβ. Estrogen receptors are also implicated in the development and progression of breast cancer. Here, we found that ERα localized on the spindle and spindle poles at the metaphase during mitosis. Depletion of ERα generated unaligned chromosomes in metaphase cells and lagging chromosomes in anaphase cells in a transcription-independent manner. Furthermore, the levels of β-tubulin and γ-tubulin were reduced in ERα-depleted cells. Consistent with this, polymerization of microtubules in ERα-depleted cells and turnover rate of α/β-tubulin were decreased than in control cells. We suggest that ERα regulates chromosome alignment and spindle dynamics by stabilizing microtubules during mitosis.

AB - Estrogen receptors are activated by the hormone estrogen and they control cell growth by altering gene expression as a transcription factor. So far two estrogen receptors have been found: ERα and ERβ. Estrogen receptors are also implicated in the development and progression of breast cancer. Here, we found that ERα localized on the spindle and spindle poles at the metaphase during mitosis. Depletion of ERα generated unaligned chromosomes in metaphase cells and lagging chromosomes in anaphase cells in a transcription-independent manner. Furthermore, the levels of β-tubulin and γ-tubulin were reduced in ERα-depleted cells. Consistent with this, polymerization of microtubules in ERα-depleted cells and turnover rate of α/β-tubulin were decreased than in control cells. We suggest that ERα regulates chromosome alignment and spindle dynamics by stabilizing microtubules during mitosis.

KW - Chromosome movement

KW - ERα

KW - Mitotic spindle

KW - Spindle dynamics

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ERα regulates chromosome alignment and spindle dynamics during mitosis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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