TY - JOUR
T1 - ERBB receptor feedback inhibitor 1
T2 - Identification and regulation by estrogen in chickens
AU - Ahn, Suzie E.
AU - Jeong, Wooyoung
AU - Kim, Ji Hye
AU - Lim, Whasun
AU - Kim, Jinyoung
AU - Bazer, Fuller W.
AU - Han, Jae Yong
AU - Song, Gwonhwa
N1 - Funding Information:
This research was funded by the World Class University (WCU) Program ( R31-10056 ) and by Basic Science Research Program ( 2010-0013078 ) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology , and also by a Grant from the Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea . We thank all members of the Laboratory of Molecular Endocrinology and Cell Signaling and Laboratory of Animal Genetic Engineering for assistance and management of animals. The authors also thank to Drs. Jae-Wook Jeong, Sang In Lee and Jin Won Choi for technical assistance and helpful discussions.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The ERBB receptor feedback inhibitor 1 (ERRFI1) is a scaffolding adaptor protein, that plays a pivotal role in the epidermal growth factor receptor (EGFR) cell signaling cascade as a negative regulator affecting many important physiological processes. It was recently reported that ERRFI1 is a critical regulator of the response of the endometrium to estrogen regulation of tissue homeostasis in mice. But, very little is known about ERRF11 and hormonal regulation of the ERRFI1 gene in chickens. Therefore, in the present study, ERRFI1 gene was cloned and its differential expression profile analyzed at different embryonic stages, in various adult organs, and in oviducts from estrogen-treated chickens. Chicken ERRFI1 has an open-reading frame of 2848 nucleotides that encode for a protein of 465 amino acids that has considerable homology to mammalian ERRFI1 proteins (>62% identity). Importantly, ERRFI1 mRNA is abundantly distributed in various organs from chickens. We then determined that DES (diethylstilbestrol, a synthetic nonsteroidal estrogen) induced ERRFI1 mRNA and protein predominantly in luminal and glandular epithelial cells of the oviduct. Further, we determined whether microRNAs, specifically miR-200b, miR-429 and miR-1639, influence ERRFI1 expression via its 3'UTR and found that it does not directly target the 3'UTR of ERRFI1 mRNA. Therefore, it is unlikely that post-transcriptional regulation influences ERRFI1 expression in the chicken oviduct. In conclusion, our results indicate that ERRFI1 is a novel estrogen-stimulated gene expressed in epithelial cells of the chicken oviduct that likely plays an important role in oviduct growth and differentiation during early development of the chicken.
AB - The ERBB receptor feedback inhibitor 1 (ERRFI1) is a scaffolding adaptor protein, that plays a pivotal role in the epidermal growth factor receptor (EGFR) cell signaling cascade as a negative regulator affecting many important physiological processes. It was recently reported that ERRFI1 is a critical regulator of the response of the endometrium to estrogen regulation of tissue homeostasis in mice. But, very little is known about ERRF11 and hormonal regulation of the ERRFI1 gene in chickens. Therefore, in the present study, ERRFI1 gene was cloned and its differential expression profile analyzed at different embryonic stages, in various adult organs, and in oviducts from estrogen-treated chickens. Chicken ERRFI1 has an open-reading frame of 2848 nucleotides that encode for a protein of 465 amino acids that has considerable homology to mammalian ERRFI1 proteins (>62% identity). Importantly, ERRFI1 mRNA is abundantly distributed in various organs from chickens. We then determined that DES (diethylstilbestrol, a synthetic nonsteroidal estrogen) induced ERRFI1 mRNA and protein predominantly in luminal and glandular epithelial cells of the oviduct. Further, we determined whether microRNAs, specifically miR-200b, miR-429 and miR-1639, influence ERRFI1 expression via its 3'UTR and found that it does not directly target the 3'UTR of ERRFI1 mRNA. Therefore, it is unlikely that post-transcriptional regulation influences ERRFI1 expression in the chicken oviduct. In conclusion, our results indicate that ERRFI1 is a novel estrogen-stimulated gene expressed in epithelial cells of the chicken oviduct that likely plays an important role in oviduct growth and differentiation during early development of the chicken.
KW - Chicken
KW - DES
KW - ERRFI1
KW - Oviduct
UR - http://www.scopus.com/inward/record.url?scp=84855204462&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2011.11.013
DO - 10.1016/j.ygcen.2011.11.013
M3 - Article
C2 - 22137914
AN - SCOPUS:84855204462
SN - 0016-6480
VL - 175
SP - 194
EP - 205
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -