Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

Jae Hong Kim, Hee Jin Kwack, Sung E. Choi, Byung Chul Kim, Yong Sun Kim, Il Jun Kang, Chandra C. Kumar

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalFEBS Letters
Volume406
Issue number1-2
DOIs
Publication statusPublished - 1997 Apr 7
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grant from Hallym Academy of Science, Hallym University, 1996 (to I.J. Kang). We wish to thank Dr. Ron. Prywes (Columbia University, USA) for providing us with SRE-luciferase plasmids. We also wish to thank Dr. Allan Hall (University College, London, UK) for providing us pEXV-Rac plasmid.

Keywords

  • Arachidonic acid
  • Hydrogen peroxide
  • Phospholipase A
  • Rac GTPase
  • Serum response element
  • c-fos

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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