Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria

Changsu Lee; Min Seo Jeon; Thi Thao Vo; Chulhwan Park; Jong Soon Choi; Joseph Kwon; Seong Woon Roh; Yoon E. Choi

doi:10.1007/s10811-018-1403-8

Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria

Changsu Lee, Min Seo Jeon, Thi Thao Vo, Chulhwan Park, Jong Soon Choi, Joseph Kwon, Seong Woon Roh, Yoon E. Choi

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Unwanted, rapid increases in the algal populations of water systems cause harmful algal blooms, which have recently become a major environmental problem. The cyanobacterium Microcystis aeruginosa is the most prevalent bloom species and is responsible for the majority of blooms in freshwater environments. In this study, we attempted to develop an eco-friendly method to suppress M. aeruginosa bloom based on a biological control using bacteria newly isolated from the soil. In a screen for bacteria with strong lethal activity toward Microcystis, we isolated Bacillus sp. T4 and characterised its algicidal activity. Microcystis aeruginosa cells were killed via indirect attack by compound(s) secreted by T4 bacteria. ELISA revealed a dramatic increase in extracellular microcystins in M. aeruginosa cultures upon treatment with T4. Therefore, we screened for bacteria that could degrade these toxins, and three new isolates (R12, S42 and S65) were identified. Simultaneous application of both T4 as a lytic agent and R12 or S42 as toxin-degrading bacteria could eliminate both Microcystis cells and its problematic toxin. Our eco-friendly approach, based on the application of newly isolated bacteria, provides a novel method to control harmful algal blooms.

Original language	English
Pages (from-to)	1795-1806
Number of pages	12
Journal	Journal of Applied Phycology
Volume	30
Issue number	3
DOIs	https://doi.org/10.1007/s10811-018-1403-8
Publication status	Published - 2018 Jun 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2016R1D1A1B03932773), the World Institute of Kimchi (KE1802-2), funded by the Ministry of Science and ICT and Korea Basic Science Institute under the R&D program (Project No. C38703) supervised by the Ministry of Science and ICT, Republic of Korea. This work was also supported by the research program of KAERI, Republic of Korea. In addition, this research was supported by Korea University Future Research Grant and Korea University (OJERI) Grant. The authors declare no conflict of interest.

Funding Information:
Acknowledgements This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2016R1D1A1B03932773), the World Institute of Kimchi (KE1802-2), funded by the Ministry of Science and ICT and Korea Basic Science Institute under the R&D program (Project No. C38703) supervised by the Ministry of Science and ICT, Republic of Korea. This work was also supported by the research program of KAERI, Republic of Korea. In addition, this research was supported by Korea University Future Research Grant and Korea University (OJERI) Grant.

Keywords

Algal bloom
Bacterial isolation
Biological control
Cyanobacterium
Microcystin degradation
Microcystis

ASJC Scopus subject areas

Aquatic Science
Plant Science

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10.1007/s10811-018-1403-8

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title = "Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria",

abstract = "Unwanted, rapid increases in the algal populations of water systems cause harmful algal blooms, which have recently become a major environmental problem. The cyanobacterium Microcystis aeruginosa is the most prevalent bloom species and is responsible for the majority of blooms in freshwater environments. In this study, we attempted to develop an eco-friendly method to suppress M. aeruginosa bloom based on a biological control using bacteria newly isolated from the soil. In a screen for bacteria with strong lethal activity toward Microcystis, we isolated Bacillus sp. T4 and characterised its algicidal activity. Microcystis aeruginosa cells were killed via indirect attack by compound(s) secreted by T4 bacteria. ELISA revealed a dramatic increase in extracellular microcystins in M. aeruginosa cultures upon treatment with T4. Therefore, we screened for bacteria that could degrade these toxins, and three new isolates (R12, S42 and S65) were identified. Simultaneous application of both T4 as a lytic agent and R12 or S42 as toxin-degrading bacteria could eliminate both Microcystis cells and its problematic toxin. Our eco-friendly approach, based on the application of newly isolated bacteria, provides a novel method to control harmful algal blooms.",

keywords = "Algal bloom, Bacterial isolation, Biological control, Cyanobacterium, Microcystin degradation, Microcystis",

author = "Changsu Lee and Jeon, {Min Seo} and Vo, {Thi Thao} and Chulhwan Park and Choi, {Jong Soon} and Joseph Kwon and Roh, {Seong Woon} and Choi, {Yoon E.}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2016R1D1A1B03932773), the World Institute of Kimchi (KE1802-2), funded by the Ministry of Science and ICT and Korea Basic Science Institute under the R&D program (Project No. C38703) supervised by the Ministry of Science and ICT, Republic of Korea. This work was also supported by the research program of KAERI, Republic of Korea. In addition, this research was supported by Korea University Future Research Grant and Korea University (OJERI) Grant. The authors declare no conflict of interest. Funding Information: Acknowledgements This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2016R1D1A1B03932773), the World Institute of Kimchi (KE1802-2), funded by the Ministry of Science and ICT and Korea Basic Science Institute under the R&D program (Project No. C38703) supervised by the Ministry of Science and ICT, Republic of Korea. This work was also supported by the research program of KAERI, Republic of Korea. In addition, this research was supported by Korea University Future Research Grant and Korea University (OJERI) Grant.",

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AU - Lee, Changsu

AU - Jeon, Min Seo

AU - Vo, Thi Thao

AU - Park, Chulhwan

AU - Choi, Jong Soon

AU - Kwon, Joseph

AU - Roh, Seong Woon

AU - Choi, Yoon E.

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2016R1D1A1B03932773), the World Institute of Kimchi (KE1802-2), funded by the Ministry of Science and ICT and Korea Basic Science Institute under the R&D program (Project No. C38703) supervised by the Ministry of Science and ICT, Republic of Korea. This work was also supported by the research program of KAERI, Republic of Korea. In addition, this research was supported by Korea University Future Research Grant and Korea University (OJERI) Grant. The authors declare no conflict of interest. Funding Information: Acknowledgements This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2016R1D1A1B03932773), the World Institute of Kimchi (KE1802-2), funded by the Ministry of Science and ICT and Korea Basic Science Institute under the R&D program (Project No. C38703) supervised by the Ministry of Science and ICT, Republic of Korea. This work was also supported by the research program of KAERI, Republic of Korea. In addition, this research was supported by Korea University Future Research Grant and Korea University (OJERI) Grant.

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N2 - Unwanted, rapid increases in the algal populations of water systems cause harmful algal blooms, which have recently become a major environmental problem. The cyanobacterium Microcystis aeruginosa is the most prevalent bloom species and is responsible for the majority of blooms in freshwater environments. In this study, we attempted to develop an eco-friendly method to suppress M. aeruginosa bloom based on a biological control using bacteria newly isolated from the soil. In a screen for bacteria with strong lethal activity toward Microcystis, we isolated Bacillus sp. T4 and characterised its algicidal activity. Microcystis aeruginosa cells were killed via indirect attack by compound(s) secreted by T4 bacteria. ELISA revealed a dramatic increase in extracellular microcystins in M. aeruginosa cultures upon treatment with T4. Therefore, we screened for bacteria that could degrade these toxins, and three new isolates (R12, S42 and S65) were identified. Simultaneous application of both T4 as a lytic agent and R12 or S42 as toxin-degrading bacteria could eliminate both Microcystis cells and its problematic toxin. Our eco-friendly approach, based on the application of newly isolated bacteria, provides a novel method to control harmful algal blooms.

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Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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