Establishment of a phagocytic cell line from Bombina orientalis

Sang Chan Park, Sang Ho Lee, Sung Sik Han

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Continuous serum-free culture of Bok-2 cells was generated from primary culture of the tail bud stage embryos of the toad, Bombina orientalis. Bok-2 cells can be maintained in modified L-15 serum-free medium prepared by mixing L-15 medium, lactalbumin enzymatic hydrolysate, sucrose and sodium bicarbonate. Bok-2 cells have an ameboid behavior and morphology. When Bok-2 and viable Candida albicans were co-cultured, Bok-2 cells showed an immune response characterized by chemotaxis, phagocytosis and partial clearing activity of Candida cells and colonies. And Bok-2 cells also showed phagocytosis of latex beads without serum treatment and displayed numerous pseudopodia, membrane evagination for phagocytosis and phagosome activity. On the basis of these results, Bok-2 cells were identified as professional phagocytes.

    Original languageEnglish
    Pages (from-to)1-7
    Number of pages7
    JournalMethods in Cell Science
    Volume22
    Issue number1
    DOIs
    Publication statusPublished - 2000 Apr

    Bibliographical note

    Funding Information:
    This work was supported in part by grant SGRP/HTDP (1996) from the Ministry of Agriculture and Forestry to S. S. Han.

    Keywords

    • Amphibian
    • Bombina orientalis
    • Cell culture
    • Phagocytosis
    • Serum-free

    ASJC Scopus subject areas

    • Cell Biology

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