Establishment of a phagocytic cell line from Bombina orientalis

Sang Chan Park, Sang Ho Lee, Sung Sik Han

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Continuous serum-free culture of Bok-2 cells was generated from primary culture of the tail bud stage embryos of the toad, Bombina orientalis. Bok-2 cells can be maintained in modified L-15 serum-free medium prepared by mixing L-15 medium, lactalbumin enzymatic hydrolysate, sucrose and sodium bicarbonate. Bok-2 cells have an ameboid behavior and morphology. When Bok-2 and viable Candida albicans were co-cultured, Bok-2 cells showed an immune response characterized by chemotaxis, phagocytosis and partial clearing activity of Candida cells and colonies. And Bok-2 cells also showed phagocytosis of latex beads without serum treatment and displayed numerous pseudopodia, membrane evagination for phagocytosis and phagosome activity. On the basis of these results, Bok-2 cells were identified as professional phagocytes.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalMethods in Cell Science
Issue number1
Publication statusPublished - 2000 Apr

Bibliographical note

Funding Information:
This work was supported in part by grant SGRP/HTDP (1996) from the Ministry of Agriculture and Forestry to S. S. Han.


  • Amphibian
  • Bombina orientalis
  • Cell culture
  • Phagocytosis
  • Serum-free

ASJC Scopus subject areas

  • Cell Biology


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