Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

SUNWOO PARK; SOO JIN PARK; HEE SU LEE; JIYEON HAM; EUN JI LEE; JUNSIK KIM; SOOMIN RYU; AERAN SEOL; WHASUN LIM; JUNG CHAN LEE; GWONHWA SONG; HEE SEUNG KIM

doi:10.21873/INVIVO.12554

Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

SUNWOO PARK, SOO JIN PARK, HEE SU LEE, JIYEON HAM, EUN JI LEE, JUNSIK KIM, SOOMIN RYU, AERAN SEOL, WHASUN LIM, JUNG CHAN LEE, GWONHWA SONG, HEE SEUNG KIM

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2 Citations (Scopus)

Abstract

Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

Original language	English
Pages (from-to)	2703-2710
Number of pages	8
Journal	In Vivo
Volume	35
Issue number	5
DOIs	https://doi.org/10.21873/INVIVO.12554
Publication status	Published - 2021 Oct

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© 2021 International Institute of Anticancer Research. All rights reserved.

Keywords

Experimental system
Intraperitoneal chemotherapy
Rat

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
Pharmacology

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10.21873/INVIVO.12554

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title = "Establishment of an experimental system for intraperitoneal chemotherapy in a rat model",

abstract = "Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.",

keywords = "Experimental system, Intraperitoneal chemotherapy, Rat",

author = "SUNWOO PARK and PARK, {SOO JIN} and LEE, {HEE SU} and JIYEON HAM and LEE, {EUN JI} and JUNSIK KIM and SOOMIN RYU and AERAN SEOL and WHASUN LIM and LEE, {JUNG CHAN} and GWONHWA SONG and KIM, {HEE SEUNG}",

year = "2021",

month = oct,

doi = "10.21873/INVIVO.12554",

language = "English",

volume = "35",

pages = "2703--2710",

journal = "In Vivo",

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T1 - Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

AU - PARK, SUNWOO

AU - PARK, SOO JIN

AU - LEE, HEE SU

AU - HAM, JIYEON

AU - LEE, EUN JI

AU - KIM, JUNSIK

AU - RYU, SOOMIN

AU - SEOL, AERAN

AU - LIM, WHASUN

AU - LEE, JUNG CHAN

AU - SONG, GWONHWA

AU - KIM, HEE SEUNG

PY - 2021/10

Y1 - 2021/10

N2 - Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

AB - Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

KW - Experimental system

KW - Intraperitoneal chemotherapy

KW - Rat

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M3 - Article

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JO - In Vivo

JF - In Vivo

IS - 5

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Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

Abstract

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Keywords

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