Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

SUNWOO PARK; SOO JIN PARK; HEE SU LEE; JIYEON HAM; EUN JI LEE; JUNSIK KIM; SOOMIN RYU; AERAN SEOL; WHASUN LIM; JUNG CHAN LEE; GWONHWA SONG; HEE SEUNG KIM

doi:10.21873/INVIVO.12554

Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

SUNWOO PARK, SOO JIN PARK, HEE SU LEE, JIYEON HAM, EUN JI LEE, JUNSIK KIM, SOOMIN RYU, AERAN SEOL, WHASUN LIM, JUNG CHAN LEE, GWONHWA SONG^*, HEE SEUNG KIM^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

Original language	English
Pages (from-to)	2703-2710
Number of pages	8
Journal	In Vivo
Volume	35
Issue number	5
DOIs	https://doi.org/10.21873/INVIVO.12554
Publication status	Published - 2021 Oct

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Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.

Keywords

Experimental system
Intraperitoneal chemotherapy
Rat

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
Pharmacology

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10.21873/INVIVO.12554

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title = "Establishment of an experimental system for intraperitoneal chemotherapy in a rat model",

abstract = "Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2\% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.",

keywords = "Experimental system, Intraperitoneal chemotherapy, Rat",

author = "SUNWOO PARK and PARK, \{SOO JIN\} and LEE, \{HEE SU\} and JIYEON HAM and LEE, \{EUN JI\} and JUNSIK KIM and SOOMIN RYU and AERAN SEOL and WHASUN LIM and LEE, \{JUNG CHAN\} and GWONHWA SONG and KIM, \{HEE SEUNG\}",

year = "2021",

month = oct,

doi = "10.21873/INVIVO.12554",

language = "English",

volume = "35",

pages = "2703--2710",

journal = "In Vivo",

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publisher = "International Institute of Anticancer Research",

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TY - JOUR

T1 - Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

AU - PARK, SUNWOO

AU - PARK, SOO JIN

AU - LEE, HEE SU

AU - HAM, JIYEON

AU - LEE, EUN JI

AU - KIM, JUNSIK

AU - RYU, SOOMIN

AU - SEOL, AERAN

AU - LIM, WHASUN

AU - LEE, JUNG CHAN

AU - SONG, GWONHWA

AU - KIM, HEE SEUNG

PY - 2021/10

Y1 - 2021/10

N2 - Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

AB - Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

KW - Experimental system

KW - Intraperitoneal chemotherapy

KW - Rat

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DO - 10.21873/INVIVO.12554

M3 - Article

C2 - 34410959

AN - SCOPUS:85113824455

SN - 0258-851X

VL - 35

SP - 2703

EP - 2710

JO - In Vivo

JF - In Vivo

IS - 5

ER -

Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

Abstract

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Keywords

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