Establishment of Immortalized Human Endometriotic Stromal Cell Line from Ectopic Lesion of a Patient with Endometriosis

Daryeon Son, Hahyun Park, Garam An, Sunwoo Park, Dong Won Hwang, Soo Jin Park, Hee Seung Kim, Whasun Lim, Seungkwon You, Gwonhwa Song

Research output: Contribution to journalArticlepeer-review

Abstract

Endometriosis is an estrogen-dependent inflammatory disease characterized by the growth of endometrial-like tissues containing endometrial stromal cells and glandular epithelium outside the uterine cavity. An insufficient response to progesterone contributes to disease progression and systemic inflammation during the pathogenesis of endometriosis. Patients with endometriosis usually experience painful symptoms, dysmenorrhea, and infertility, which contribute to a significant reduction in their quality of life. To determine the possible molecular mechanisms of endometriosis and explore novel therapeutic targets, we derived primary human ovarian endometriotic stromal cells (hOESCs) from a patient of reproductive age with ovarian endometriosis. In this study, we successfully established immortalized human ovarian endometriotic stromal cell lines (ihOESCs) using primary stromal cells obtained from endometriotic lesions to overcome short lifespan and growth inhibition. Immortalization of hOESCs with human telomerase reverse transcriptase (hTERT) transfection led to cells that maintained a proliferative state under passage culture conditions without mutagenesis during cellular senescence. The morphology and karyotype of ihOESCs were unchanged compared with those of hOESCs. Moreover, ihOESCs were continuously positive for vimentin and negative for E-cadherin expression. Following decidual stimuli and inflammatory responses, both hOESCs and ihOESCs sensitively express decidualization markers and proinflammatory cytokines. Collectively, we characterized ihOESCs to maintain their phenotypic and functional properties with a longer lifespan and normal physiological responses than those of hOESCs. These immortalized cells could aid in a detailed understanding of the pathological mechanisms of endometriosis.

Original languageEnglish
Pages (from-to)2703-2714
Number of pages12
JournalReproductive Sciences
Volume30
Issue number9
DOIs
Publication statusPublished - 2023 Sept

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Society for Reproductive Investigation.

Keywords

  • Decidualization
  • Endometriosis
  • Endometriotic stromal cells
  • Immortalization
  • Inflammation
  • Steroid hormones

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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