Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)

Sanghyun Park, Jeong Eun Yoo, Dongjin R. Lee, Jiho Jang, Myung Soo Cho, Dae Sung Kim, Dong Wook Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation.

Original languageEnglish
Article number101499
JournalStem Cell Research
Volume39
DOIs
Publication statusPublished - 2019 Aug

Bibliographical note

Funding Information:
This work was supported by 1) the Bio & Medical Technology Development Program of the National Research Foundation (NRF) ( 2017M3A9B4042580 ), 2) the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare , Republic of Korea ( HI18C0829 ), 3) a Korea University Research Grant, and 4) BK21 PLUS Program for Biotechnology , Korea University .

Funding Information:
This work was supported by 1) the Bio & Medical Technology Development Program of the National Research Foundation (NRF) (2017M3A9B4042580), 2) the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI18C0829), 3) a Korea University Research Grant, and 4) BK21 PLUS Program for Biotechnology, Korea University.

Publisher Copyright:
© 2019 The Authors

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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