Ethanol production from lignocellulosic hydrolysates using engineered Saccharomyces cerevisiae harboring xylose isomerase-based pathway

Ja Kyong Ko; Youngsoon Um; Han Min Woo; Kyoung Heon Kim; Sun Mi Lee

doi:10.1016/j.biortech.2016.02.124

Ethanol production from lignocellulosic hydrolysates using engineered Saccharomyces cerevisiae harboring xylose isomerase-based pathway

Ja Kyong Ko, Youngsoon Um, Han Min Woo, Kyoung Heon Kim, Sun Mi Lee

Research output: Contribution to journal › Article › peer-review

105 Citations (Scopus)

Abstract

The efficient co-fermentation of glucose and xylose is necessary for the economically feasible bioethanol production from lignocellulosic biomass. Even with xylose utilizing Saccharomyces cerevisiae, the efficiency of the lignocellulosic ethanol production remains suboptimal mainly due to the low conversion yield of xylose to ethanol. In this study, we evaluated the co-fermentation performances of SXA-R2P-E, a recently engineered isomerase-based xylose utilizing strain, in mixed sugars and in lignocellulosic hydrolysates. In a high-sugar fermentation with 70 g/L of glucose and 40 g/L of xylose, SXA-R2P-E produced 50 g/L of ethanol with an yield of 0.43 g ethanol/g sugars at 72 h. From dilute acid-pretreated hydrolysates of rice straw and hardwood (oak), the strain produced 18-21 g/L of ethanol with among the highest yield of 0.43-0.46 g ethanol/g sugars ever reported. This study shows a highly promising potential of a xylose isomerase-expressing strain as an industrially relevant ethanol producer from lignocellulosic hydrolysates.

Original language	English
Pages (from-to)	290-296
Number of pages	7
Journal	Bioresource technology
Volume	209
DOIs	https://doi.org/10.1016/j.biortech.2016.02.124
Publication status	Published - 2016 Jun 1

Bibliographical note

Funding Information:
The authors acknowledge Dr. Hal S. Alper at The University of Texas at Austin for providing the strain of SXA-R2P-E. This research was supported by the R&D Convergence Program of NST ( National Research Council of Science & Technology ) of Republic of Korea/Korea Institute of Science and Technology ( KIST ), and the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) granted financial resource from the Ministry of Trade, Industry & Energy , Republic of Korea (No. 20153030091360 ).

Publisher Copyright:
© 2016 Elsevier Ltd.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

Co-fermentation
Ethanol
Lignocellulosic hydrolysate
Saccharomyces cerevisiae
Xylose isomerase

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2016.02.124

Cite this

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title = "Ethanol production from lignocellulosic hydrolysates using engineered Saccharomyces cerevisiae harboring xylose isomerase-based pathway",

abstract = "The efficient co-fermentation of glucose and xylose is necessary for the economically feasible bioethanol production from lignocellulosic biomass. Even with xylose utilizing Saccharomyces cerevisiae, the efficiency of the lignocellulosic ethanol production remains suboptimal mainly due to the low conversion yield of xylose to ethanol. In this study, we evaluated the co-fermentation performances of SXA-R2P-E, a recently engineered isomerase-based xylose utilizing strain, in mixed sugars and in lignocellulosic hydrolysates. In a high-sugar fermentation with 70 g/L of glucose and 40 g/L of xylose, SXA-R2P-E produced 50 g/L of ethanol with an yield of 0.43 g ethanol/g sugars at 72 h. From dilute acid-pretreated hydrolysates of rice straw and hardwood (oak), the strain produced 18-21 g/L of ethanol with among the highest yield of 0.43-0.46 g ethanol/g sugars ever reported. This study shows a highly promising potential of a xylose isomerase-expressing strain as an industrially relevant ethanol producer from lignocellulosic hydrolysates.",

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note = "Funding Information: The authors acknowledge Dr. Hal S. Alper at The University of Texas at Austin for providing the strain of SXA-R2P-E. This research was supported by the R&D Convergence Program of NST ( National Research Council of Science & Technology ) of Republic of Korea/Korea Institute of Science and Technology ( KIST ), and the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) granted financial resource from the Ministry of Trade, Industry & Energy , Republic of Korea (No. 20153030091360 ). Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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AU - Lee, Sun Mi

N1 - Funding Information: The authors acknowledge Dr. Hal S. Alper at The University of Texas at Austin for providing the strain of SXA-R2P-E. This research was supported by the R&D Convergence Program of NST ( National Research Council of Science & Technology ) of Republic of Korea/Korea Institute of Science and Technology ( KIST ), and the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) granted financial resource from the Ministry of Trade, Industry & Energy , Republic of Korea (No. 20153030091360 ). Publisher Copyright: © 2016 Elsevier Ltd. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

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N2 - The efficient co-fermentation of glucose and xylose is necessary for the economically feasible bioethanol production from lignocellulosic biomass. Even with xylose utilizing Saccharomyces cerevisiae, the efficiency of the lignocellulosic ethanol production remains suboptimal mainly due to the low conversion yield of xylose to ethanol. In this study, we evaluated the co-fermentation performances of SXA-R2P-E, a recently engineered isomerase-based xylose utilizing strain, in mixed sugars and in lignocellulosic hydrolysates. In a high-sugar fermentation with 70 g/L of glucose and 40 g/L of xylose, SXA-R2P-E produced 50 g/L of ethanol with an yield of 0.43 g ethanol/g sugars at 72 h. From dilute acid-pretreated hydrolysates of rice straw and hardwood (oak), the strain produced 18-21 g/L of ethanol with among the highest yield of 0.43-0.46 g ethanol/g sugars ever reported. This study shows a highly promising potential of a xylose isomerase-expressing strain as an industrially relevant ethanol producer from lignocellulosic hydrolysates.

AB - The efficient co-fermentation of glucose and xylose is necessary for the economically feasible bioethanol production from lignocellulosic biomass. Even with xylose utilizing Saccharomyces cerevisiae, the efficiency of the lignocellulosic ethanol production remains suboptimal mainly due to the low conversion yield of xylose to ethanol. In this study, we evaluated the co-fermentation performances of SXA-R2P-E, a recently engineered isomerase-based xylose utilizing strain, in mixed sugars and in lignocellulosic hydrolysates. In a high-sugar fermentation with 70 g/L of glucose and 40 g/L of xylose, SXA-R2P-E produced 50 g/L of ethanol with an yield of 0.43 g ethanol/g sugars at 72 h. From dilute acid-pretreated hydrolysates of rice straw and hardwood (oak), the strain produced 18-21 g/L of ethanol with among the highest yield of 0.43-0.46 g ethanol/g sugars ever reported. This study shows a highly promising potential of a xylose isomerase-expressing strain as an industrially relevant ethanol producer from lignocellulosic hydrolysates.

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ER -

Ethanol production from lignocellulosic hydrolysates using engineered Saccharomyces cerevisiae harboring xylose isomerase-based pathway

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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