Evaluation and optimization of quantitative analysis of cofactors from yeast by liquid chromatography/mass spectrometry

Jungyeon Kim; Inho Jung; Yu Eun Cheong; Kyoung Heon Kim

doi:10.1016/j.aca.2022.339890

Evaluation and optimization of quantitative analysis of cofactors from yeast by liquid chromatography/mass spectrometry

Jungyeon Kim, Inho Jung, Yu Eun Cheong, Kyoung Heon Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Cofactors play pivotal roles in catabolism and anabolism in all living organisms. Many studies have investigated the concentration of cofactors in living organisms to understand their metabolic status, which can be used to produce valuable chemicals or to understand the pathophysiology of diseases. Among various analytical platforms, liquid chromatography/mass spectrometry (LC/MS) is the most frequently used method for the quantification of cofactors. Several studies have reported various analytical methods for cofactors using LC/MS. However, the lack of optimal LC/MS methods makes it challenging to analyze various cofactors simultaneously. In addition, the method of extracting cofactors from cells needs to be optimized because conventional protocols probably have low extraction efficiency, which makes it difficult to reflect the actual concentration of cofactors in cells. In this study, we systematically compared various analytical methods and suggested optimal methods for the analysis of cofactors using LC/MS. In addition, we systematically compared quenching methods and extraction solvents and suggested optimal methods for the extraction of cofactors from Saccharomyces cerevisiae. The optimized methods can be used as standard protocols for LC/MS analysis and the extraction of cofactors from S. cerevisiae.

Original language	English
Article number	339890
Journal	Analytica Chimica Acta
Volume	1211
DOIs	https://doi.org/10.1016/j.aca.2022.339890
Publication status	Published - 2022 Jun 8

Bibliographical note

Funding Information:
This work was supported by the Mid-career Researcher Program through the National Research Foundation (NRF) of Korea ( 2020R1A2B5B02002631 ) and by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries, funded by the Ministry of Agriculture, Food and Rural Affairs ( 32136-05-1-SB010 ). This work was also supported by the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety and by a Korea University Grant.

Publisher Copyright:
© 2022

Keywords

Cofactors
Liquid chromatography/mass spectrometry
Metabolome
Saccharomyces cerevisiae
Yeast

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/j.aca.2022.339890

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title = "Evaluation and optimization of quantitative analysis of cofactors from yeast by liquid chromatography/mass spectrometry",

abstract = "Cofactors play pivotal roles in catabolism and anabolism in all living organisms. Many studies have investigated the concentration of cofactors in living organisms to understand their metabolic status, which can be used to produce valuable chemicals or to understand the pathophysiology of diseases. Among various analytical platforms, liquid chromatography/mass spectrometry (LC/MS) is the most frequently used method for the quantification of cofactors. Several studies have reported various analytical methods for cofactors using LC/MS. However, the lack of optimal LC/MS methods makes it challenging to analyze various cofactors simultaneously. In addition, the method of extracting cofactors from cells needs to be optimized because conventional protocols probably have low extraction efficiency, which makes it difficult to reflect the actual concentration of cofactors in cells. In this study, we systematically compared various analytical methods and suggested optimal methods for the analysis of cofactors using LC/MS. In addition, we systematically compared quenching methods and extraction solvents and suggested optimal methods for the extraction of cofactors from Saccharomyces cerevisiae. The optimized methods can be used as standard protocols for LC/MS analysis and the extraction of cofactors from S. cerevisiae.",

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author = "Jungyeon Kim and Inho Jung and Cheong, {Yu Eun} and Kim, {Kyoung Heon}",

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N2 - Cofactors play pivotal roles in catabolism and anabolism in all living organisms. Many studies have investigated the concentration of cofactors in living organisms to understand their metabolic status, which can be used to produce valuable chemicals or to understand the pathophysiology of diseases. Among various analytical platforms, liquid chromatography/mass spectrometry (LC/MS) is the most frequently used method for the quantification of cofactors. Several studies have reported various analytical methods for cofactors using LC/MS. However, the lack of optimal LC/MS methods makes it challenging to analyze various cofactors simultaneously. In addition, the method of extracting cofactors from cells needs to be optimized because conventional protocols probably have low extraction efficiency, which makes it difficult to reflect the actual concentration of cofactors in cells. In this study, we systematically compared various analytical methods and suggested optimal methods for the analysis of cofactors using LC/MS. In addition, we systematically compared quenching methods and extraction solvents and suggested optimal methods for the extraction of cofactors from Saccharomyces cerevisiae. The optimized methods can be used as standard protocols for LC/MS analysis and the extraction of cofactors from S. cerevisiae.

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Evaluation and optimization of quantitative analysis of cofactors from yeast by liquid chromatography/mass spectrometry

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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