Cofactors play pivotal roles in catabolism and anabolism in all living organisms. Many studies have investigated the concentration of cofactors in living organisms to understand their metabolic status, which can be used to produce valuable chemicals or to understand the pathophysiology of diseases. Among various analytical platforms, liquid chromatography/mass spectrometry (LC/MS) is the most frequently used method for the quantification of cofactors. Several studies have reported various analytical methods for cofactors using LC/MS. However, the lack of optimal LC/MS methods makes it challenging to analyze various cofactors simultaneously. In addition, the method of extracting cofactors from cells needs to be optimized because conventional protocols probably have low extraction efficiency, which makes it difficult to reflect the actual concentration of cofactors in cells. In this study, we systematically compared various analytical methods and suggested optimal methods for the analysis of cofactors using LC/MS. In addition, we systematically compared quenching methods and extraction solvents and suggested optimal methods for the extraction of cofactors from Saccharomyces cerevisiae. The optimized methods can be used as standard protocols for LC/MS analysis and the extraction of cofactors from S. cerevisiae.
Bibliographical noteFunding Information:
This work was supported by the Mid-career Researcher Program through the National Research Foundation (NRF) of Korea ( 2020R1A2B5B02002631 ) and by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries, funded by the Ministry of Agriculture, Food and Rural Affairs ( 32136-05-1-SB010 ). This work was also supported by the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety and by a Korea University Grant.
- Liquid chromatography/mass spectrometry
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Analytical Chemistry
- Environmental Chemistry