Evaluation of antitumor metastasis via immunostimulating activities of pectic polysaccharides isolated from radish leaves

Seung U. Son; Ho Young Park; Hyung Joo Suh; Kwang Soon Shin

doi:10.1016/j.jff.2021.104639

Evaluation of antitumor metastasis via immunostimulating activities of pectic polysaccharides isolated from radish leaves

Seung U. Son, Ho Young Park, Hyung Joo Suh, Kwang Soon Shin

School of Biosystem and Biomedical Science

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We investigated the antimetastatic effects of polysaccharides (RWP) isolated from radish leaves by hot water extraction and ethanol precipitation. From the sugar composition analysis, RWP was estimated to be a pectic polysaccharide. Peritoneal macrophages stimulated with RWP significantly augmented the production of various cytokines. In an assay for natural killer (NK) cell activity, both intravenous (i.v.) and oral administration of RWP significantly enhanced NK cells cytotoxicity against YAC-1 lymphoma. In an experiment on lung metastasis of Colon26-M3.1 carcinoma, prophylactic i.v. and oral administration of RWP inhibited metastasis by 77.2% and 55.0%, respectively, at 1000 μg/mouse. Furthermore, pretreatment with anti-asialo GM1 for NK cells depletion partly suppressed the inhibitory effect of RWP on lung metastasis. Thus, our study results suggest that RWP has superior antimetastatic activity, and this effect is related to the activation of various immune factors.

Original language	English
Article number	104639
Journal	Journal of Functional Foods
Volume	85
DOIs	https://doi.org/10.1016/j.jff.2021.104639
Publication status	Published - 2021 Oct

Keywords

Anti-metastasis
Macrophage activation
NK cell cytotoxicity
Radish leaf polysaccharide

ASJC Scopus subject areas

Food Science
Medicine (miscellaneous)
Nutrition and Dietetics

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10.1016/j.jff.2021.104639

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title = "Evaluation of antitumor metastasis via immunostimulating activities of pectic polysaccharides isolated from radish leaves",

abstract = "We investigated the antimetastatic effects of polysaccharides (RWP) isolated from radish leaves by hot water extraction and ethanol precipitation. From the sugar composition analysis, RWP was estimated to be a pectic polysaccharide. Peritoneal macrophages stimulated with RWP significantly augmented the production of various cytokines. In an assay for natural killer (NK) cell activity, both intravenous (i.v.) and oral administration of RWP significantly enhanced NK cells cytotoxicity against YAC-1 lymphoma. In an experiment on lung metastasis of Colon26-M3.1 carcinoma, prophylactic i.v. and oral administration of RWP inhibited metastasis by 77.2% and 55.0%, respectively, at 1000 μg/mouse. Furthermore, pretreatment with anti-asialo GM1 for NK cells depletion partly suppressed the inhibitory effect of RWP on lung metastasis. Thus, our study results suggest that RWP has superior antimetastatic activity, and this effect is related to the activation of various immune factors.",

keywords = "Anti-metastasis, Macrophage activation, NK cell cytotoxicity, Radish leaf polysaccharide",

author = "Son, {Seung U.} and Park, {Ho Young} and Suh, {Hyung Joo} and Shin, {Kwang Soon}",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

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doi = "10.1016/j.jff.2021.104639",

language = "English",

volume = "85",

journal = "Journal of Functional Foods",

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T1 - Evaluation of antitumor metastasis via immunostimulating activities of pectic polysaccharides isolated from radish leaves

AU - Son, Seung U.

AU - Park, Ho Young

AU - Suh, Hyung Joo

AU - Shin, Kwang Soon

PY - 2021/10

Y1 - 2021/10

N2 - We investigated the antimetastatic effects of polysaccharides (RWP) isolated from radish leaves by hot water extraction and ethanol precipitation. From the sugar composition analysis, RWP was estimated to be a pectic polysaccharide. Peritoneal macrophages stimulated with RWP significantly augmented the production of various cytokines. In an assay for natural killer (NK) cell activity, both intravenous (i.v.) and oral administration of RWP significantly enhanced NK cells cytotoxicity against YAC-1 lymphoma. In an experiment on lung metastasis of Colon26-M3.1 carcinoma, prophylactic i.v. and oral administration of RWP inhibited metastasis by 77.2% and 55.0%, respectively, at 1000 μg/mouse. Furthermore, pretreatment with anti-asialo GM1 for NK cells depletion partly suppressed the inhibitory effect of RWP on lung metastasis. Thus, our study results suggest that RWP has superior antimetastatic activity, and this effect is related to the activation of various immune factors.

AB - We investigated the antimetastatic effects of polysaccharides (RWP) isolated from radish leaves by hot water extraction and ethanol precipitation. From the sugar composition analysis, RWP was estimated to be a pectic polysaccharide. Peritoneal macrophages stimulated with RWP significantly augmented the production of various cytokines. In an assay for natural killer (NK) cell activity, both intravenous (i.v.) and oral administration of RWP significantly enhanced NK cells cytotoxicity against YAC-1 lymphoma. In an experiment on lung metastasis of Colon26-M3.1 carcinoma, prophylactic i.v. and oral administration of RWP inhibited metastasis by 77.2% and 55.0%, respectively, at 1000 μg/mouse. Furthermore, pretreatment with anti-asialo GM1 for NK cells depletion partly suppressed the inhibitory effect of RWP on lung metastasis. Thus, our study results suggest that RWP has superior antimetastatic activity, and this effect is related to the activation of various immune factors.

KW - Anti-metastasis

KW - Macrophage activation

KW - NK cell cytotoxicity

KW - Radish leaf polysaccharide

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U2 - 10.1016/j.jff.2021.104639

DO - 10.1016/j.jff.2021.104639

M3 - Article

AN - SCOPUS:85111057286

SN - 1756-4646

VL - 85

JO - Journal of Functional Foods

JF - Journal of Functional Foods

M1 - 104639

ER -

Evaluation of antitumor metastasis via immunostimulating activities of pectic polysaccharides isolated from radish leaves

Abstract

Keywords

ASJC Scopus subject areas

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