TY - GEN
T1 - Evaluation of brain-blood barrier permeable peptide using microfluidic reconstituted BBB model
AU - Chung, Bohye
AU - Kim, Jaehoon
AU - Kim, Hyunho
AU - Oh, Hyun Jeong
AU - Chung, Seok
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The Blood-brain barrier (BBB) is a selective barrier blocking the passage of most molecules for the central nervous system (CNS). Because of unique function of blood-brain barrier, the development of therapeutic drugs for brain diseases and evaluating of the CNS drug candidates has been interrupted. To develop a useful in vitro BBB model, we constructed a microfluidic BBB model. We compared the permeability value of hCMEC/D3 monolayer with hMVEC monolayer. Furthermore, we applied a cell-penetrating peptide, Angiopep-2, to our BBB platform. The higher permeability value of Angiopep-2 than same sized dextran molecule was proved in BBB device.
AB - The Blood-brain barrier (BBB) is a selective barrier blocking the passage of most molecules for the central nervous system (CNS). Because of unique function of blood-brain barrier, the development of therapeutic drugs for brain diseases and evaluating of the CNS drug candidates has been interrupted. To develop a useful in vitro BBB model, we constructed a microfluidic BBB model. We compared the permeability value of hCMEC/D3 monolayer with hMVEC monolayer. Furthermore, we applied a cell-penetrating peptide, Angiopep-2, to our BBB platform. The higher permeability value of Angiopep-2 than same sized dextran molecule was proved in BBB device.
KW - Brain-blood barrier
KW - Cell penetrating peptide
KW - Microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85079780105&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079780105&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1601
EP - 1603
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -