Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides

Dong Kyu Chae, Eunmi Ban, Young Sook Yoo, Ja‐Hyun ‐H Baik, Eun Joo Song

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    MicroRNAs (miRNAs) are short RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play an important role in the pathogenesis of diseases. Numerous studies aimed at developing novel miRNA-based drugs or determining miRNA functions have been conducted by inhibiting miRNAs using anti-miRNA oligonucleotides (AMOs), which inhibit the function by hybridizing with miRNA. To increase the binding affinity and specificity to target miRNA, AMOs with various chemical modifications have been developed. Evaluating the potency of these various types of AMOs is an essential step in their development. In this study, we developed a capillary electrophoresis with laser-induced fluorescence (CE-LIF) method to evaluate the potency of AMOs by measuring changes in miRNA levels with fluorescence-labeled ssDNA probes using AMO-miR-23a, which inhibits miR-23a related to lung cancer. In order to eliminate interference by excess AMOs during hybridization of the ssDNA probe with the miR-23a, the concentration of the ssDNA probe was optimized. This newly developed method was used to compare the potency of two different modified AMOs. The data were supported by the results of a luciferase assay. This study demonstrated that CE-LIF analysis could be used to accurately evaluate AMO potency in biological samples.

    Original languageEnglish
    Pages (from-to)4829-4833
    Number of pages5
    JournalAnalytical and Bioanalytical Chemistry
    Volume408
    Issue number18
    DOIs
    Publication statusPublished - 2016 Jul 1

    Bibliographical note

    Funding Information:
    This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (20110021713 and 2015R1A2A2A04005596) and an intramural grant from the Korea Institute of Science and Technology (2E26110).

    Publisher Copyright:
    © 2016, Springer-Verlag Berlin Heidelberg.

    Keywords

    • Anti-miRNA oligonucleotide
    • Capillary electrophoresis with laser-induced fluorescence
    • miR-23a
    • miRNA

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry

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