Evaluation of phytochemicals in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze at different growth stages by UPLC-QTof-MS

Ju Hyeon An; Heung Joo Yuk; Doo Young Kim; Chu Won Nho; Dongho Lee; Hyung Won Ryu; Sei Ryang Oh

doi:10.1016/j.indcrop.2017.12.050

Evaluation of phytochemicals in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze at different growth stages by UPLC-QTof-MS

Ju Hyeon An, Heung Joo Yuk, Doo Young Kim, Chu Won Nho, Dongho Lee, Hyung Won Ryu, Sei Ryang Oh

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

To improve the utility of A. rugosa as a seasonal edible vegetable, it is necessary to determine the components and contents of reference substances during its different growth stages. Standard markers were characterized as six compounds including a new compound 5 (acacetin 2″-O-acetyl-6″-O-malonyl-β-D-glucopyranoside) by NMR and UPLC-QTof-MS analyses. A method for qualitative/quantitative analysis of the extract was evaluated including compounds (3–5), which are easily converted to tilianin (2) during extraction & isolation process by using UPLC-QTof-MS. The contents of each compound were remarkably changed at different growth stages, especially tilianin group (2–4, and 5), which were the predominant substances (>95%) in all stages. Additionally, more comprehensive chemical information, including the relative retention time, peak area and contents, were obtained simultaneously from the raw material using marker compounds. Therefore, application of this analysis method may relieve the increasing demand for pure standards.

Original language	English
Pages (from-to)	608-616
Number of pages	9
Journal	Industrial Crops and Products
Volume	112
DOIs	https://doi.org/10.1016/j.indcrop.2017.12.050
Publication status	Published - 2018 Feb

Keywords

Acacetin derivatives
Agastache rugosa
Growth stages
Quality assurance

ASJC Scopus subject areas

Agronomy and Crop Science

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10.1016/j.indcrop.2017.12.050

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title = "Evaluation of phytochemicals in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze at different growth stages by UPLC-QTof-MS",

abstract = "To improve the utility of A. rugosa as a seasonal edible vegetable, it is necessary to determine the components and contents of reference substances during its different growth stages. Standard markers were characterized as six compounds including a new compound 5 (acacetin 2″-O-acetyl-6″-O-malonyl-β-D-glucopyranoside) by NMR and UPLC-QTof-MS analyses. A method for qualitative/quantitative analysis of the extract was evaluated including compounds (3–5), which are easily converted to tilianin (2) during extraction & isolation process by using UPLC-QTof-MS. The contents of each compound were remarkably changed at different growth stages, especially tilianin group (2–4, and 5), which were the predominant substances (>95%) in all stages. Additionally, more comprehensive chemical information, including the relative retention time, peak area and contents, were obtained simultaneously from the raw material using marker compounds. Therefore, application of this analysis method may relieve the increasing demand for pure standards.",

keywords = "Acacetin derivatives, Agastache rugosa, Growth stages, Quality assurance",

author = "An, {Ju Hyeon} and Yuk, {Heung Joo} and Kim, {Doo Young} and Nho, {Chu Won} and Dongho Lee and Ryu, {Hyung Won} and Oh, {Sei Ryang}",

note = "Funding Information: This study was supported by grants from Korea Research Institute of Bioscience and Biotechnology Research Initiative Program ( KGM1221713 ). Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2018",

month = feb,

doi = "10.1016/j.indcrop.2017.12.050",

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AU - An, Ju Hyeon

AU - Yuk, Heung Joo

AU - Kim, Doo Young

AU - Nho, Chu Won

AU - Lee, Dongho

AU - Ryu, Hyung Won

AU - Oh, Sei Ryang

N1 - Funding Information: This study was supported by grants from Korea Research Institute of Bioscience and Biotechnology Research Initiative Program ( KGM1221713 ). Publisher Copyright: © 2017 Elsevier B.V.

PY - 2018/2

Y1 - 2018/2

N2 - To improve the utility of A. rugosa as a seasonal edible vegetable, it is necessary to determine the components and contents of reference substances during its different growth stages. Standard markers were characterized as six compounds including a new compound 5 (acacetin 2″-O-acetyl-6″-O-malonyl-β-D-glucopyranoside) by NMR and UPLC-QTof-MS analyses. A method for qualitative/quantitative analysis of the extract was evaluated including compounds (3–5), which are easily converted to tilianin (2) during extraction & isolation process by using UPLC-QTof-MS. The contents of each compound were remarkably changed at different growth stages, especially tilianin group (2–4, and 5), which were the predominant substances (>95%) in all stages. Additionally, more comprehensive chemical information, including the relative retention time, peak area and contents, were obtained simultaneously from the raw material using marker compounds. Therefore, application of this analysis method may relieve the increasing demand for pure standards.

AB - To improve the utility of A. rugosa as a seasonal edible vegetable, it is necessary to determine the components and contents of reference substances during its different growth stages. Standard markers were characterized as six compounds including a new compound 5 (acacetin 2″-O-acetyl-6″-O-malonyl-β-D-glucopyranoside) by NMR and UPLC-QTof-MS analyses. A method for qualitative/quantitative analysis of the extract was evaluated including compounds (3–5), which are easily converted to tilianin (2) during extraction & isolation process by using UPLC-QTof-MS. The contents of each compound were remarkably changed at different growth stages, especially tilianin group (2–4, and 5), which were the predominant substances (>95%) in all stages. Additionally, more comprehensive chemical information, including the relative retention time, peak area and contents, were obtained simultaneously from the raw material using marker compounds. Therefore, application of this analysis method may relieve the increasing demand for pure standards.

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KW - Quality assurance

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ER -

Evaluation of phytochemicals in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze at different growth stages by UPLC-QTof-MS

Abstract

Keywords

ASJC Scopus subject areas

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