Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses

Chi Hyun Cho; Chang Kyu Lee; Myung Hyun Nam; Soo Young Yoon; Chae Seung Lim; Yunjung Cho; Young Kee Kim

doi:10.1016/j.diagmicrobio.2014.01.016

Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses

Chi Hyun Cho, Chang Kyu Lee, Myung Hyun Nam, Soo Young Yoon, Chae Seung Lim, Yunjung Cho, Young Kee Kim

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Abstract

Recently, AdvanSure™ kit based on multiplex real-time PCR was developed for simultaneous detection of 14 respiratory viruses (RVs). We compared the performance of AdvanSure with those of Seeplex® RV 15 ACE and culture by determining their sensitivities and specificities against a composite reference standard. Four hundred thirty-seven respiratory samples were tested by modified shell vial culture method, RV 15 ACE, and AdvanSure. One hundred fourteen samples (26.2%) out of 437 samples were positive by culture, while additional 91 (20.8%) were positive by AdvanSure or RV15. One hundred twelve of 114 culture-positive samples were positive by AdvanSure except 2 samples (1 adenovirus, 1 respiratory syncytial virus [RSV]). Overall, the sensitivities of culture, RV15, and AdvanSure were 74.5%, 89.8%, and 95.1%, respectively. Sensitivities of culture, RV15, and AdvanSure for each virus tested were as follows: 91/100/96% for influenza A, 60/0/100% for influenza B, 63/95/97% for RSV, 69/81/89% for adenovirus, and 87/93/93% for parainfluenza virus. For viruses not covered by culture, sensitivities of RV15 and AdvanSure were as follows: 77/88% for rhinovirus, 100/100% for coronavirus OC43, 40/100% for coronavirus 229E/NL63, 13/100% for metapneumovirus, and 44/100% for bocavirus. The overall specificities of culture, RV15, and AdvanSure were 100/98.9/99.5%, respectively. Of 45 coinfected specimens, AdvanSure detected 41 specimens (91.1%) as coinfected, while RV15 detected 27 specimens (60.0%) as coinfected. AdvanSure assay demonstrated exquisite performance for the detection of RVs and will be a valuable tool for the management of RV infection.

Original language	English
Pages (from-to)	14-18
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	79
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2014.01.016
Publication status	Published - 2014 May

Bibliographical note

Funding Information:
Funding: This study was partly supported by a grant of the Transgovernmental Enterprise for Pandemic Influenza in Korea ‚ which was the part of Korea Healthcare technology R&D Project by Ministry of Health & Welfare‚ Republic of Korea (grant no.: A103001 ).

Keywords

Multiplex real-time PCR
Respiratory virus
Viral isolation

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2014.01.016

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title = "Evaluation of the AdvanSure{\texttrademark} real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses",

abstract = "Recently, AdvanSure{\texttrademark} kit based on multiplex real-time PCR was developed for simultaneous detection of 14 respiratory viruses (RVs). We compared the performance of AdvanSure with those of Seeplex{\textregistered} RV 15 ACE and culture by determining their sensitivities and specificities against a composite reference standard. Four hundred thirty-seven respiratory samples were tested by modified shell vial culture method, RV 15 ACE, and AdvanSure. One hundred fourteen samples (26.2%) out of 437 samples were positive by culture, while additional 91 (20.8%) were positive by AdvanSure or RV15. One hundred twelve of 114 culture-positive samples were positive by AdvanSure except 2 samples (1 adenovirus, 1 respiratory syncytial virus [RSV]). Overall, the sensitivities of culture, RV15, and AdvanSure were 74.5%, 89.8%, and 95.1%, respectively. Sensitivities of culture, RV15, and AdvanSure for each virus tested were as follows: 91/100/96% for influenza A, 60/0/100% for influenza B, 63/95/97% for RSV, 69/81/89% for adenovirus, and 87/93/93% for parainfluenza virus. For viruses not covered by culture, sensitivities of RV15 and AdvanSure were as follows: 77/88% for rhinovirus, 100/100% for coronavirus OC43, 40/100% for coronavirus 229E/NL63, 13/100% for metapneumovirus, and 44/100% for bocavirus. The overall specificities of culture, RV15, and AdvanSure were 100/98.9/99.5%, respectively. Of 45 coinfected specimens, AdvanSure detected 41 specimens (91.1%) as coinfected, while RV15 detected 27 specimens (60.0%) as coinfected. AdvanSure assay demonstrated exquisite performance for the detection of RVs and will be a valuable tool for the management of RV infection.",

keywords = "Multiplex real-time PCR, Respiratory virus, Viral isolation",

author = "Cho, {Chi Hyun} and Lee, {Chang Kyu} and Nam, {Myung Hyun} and Yoon, {Soo Young} and Lim, {Chae Seung} and Yunjung Cho and Kim, {Young Kee}",

note = "Funding Information: Funding: This study was partly supported by a grant of the Transgovernmental Enterprise for Pandemic Influenza in Korea ‚ which was the part of Korea Healthcare technology R&D Project by Ministry of Health & Welfare‚ Republic of Korea (grant no.: A103001 ). ",

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AU - Nam, Myung Hyun

AU - Yoon, Soo Young

AU - Lim, Chae Seung

AU - Cho, Yunjung

AU - Kim, Young Kee

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Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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