Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses

Chi Hyun Cho, Chang Kyu Lee, Myung Hyun Nam, Soo Young Yoon, Chae Seung Lim, Yunjung Cho, Young Kee Kim

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Recently, AdvanSure™ kit based on multiplex real-time PCR was developed for simultaneous detection of 14 respiratory viruses (RVs). We compared the performance of AdvanSure with those of Seeplex® RV 15 ACE and culture by determining their sensitivities and specificities against a composite reference standard. Four hundred thirty-seven respiratory samples were tested by modified shell vial culture method, RV 15 ACE, and AdvanSure. One hundred fourteen samples (26.2%) out of 437 samples were positive by culture, while additional 91 (20.8%) were positive by AdvanSure or RV15. One hundred twelve of 114 culture-positive samples were positive by AdvanSure except 2 samples (1 adenovirus, 1 respiratory syncytial virus [RSV]). Overall, the sensitivities of culture, RV15, and AdvanSure were 74.5%, 89.8%, and 95.1%, respectively. Sensitivities of culture, RV15, and AdvanSure for each virus tested were as follows: 91/100/96% for influenza A, 60/0/100% for influenza B, 63/95/97% for RSV, 69/81/89% for adenovirus, and 87/93/93% for parainfluenza virus. For viruses not covered by culture, sensitivities of RV15 and AdvanSure were as follows: 77/88% for rhinovirus, 100/100% for coronavirus OC43, 40/100% for coronavirus 229E/NL63, 13/100% for metapneumovirus, and 44/100% for bocavirus. The overall specificities of culture, RV15, and AdvanSure were 100/98.9/99.5%, respectively. Of 45 coinfected specimens, AdvanSure detected 41 specimens (91.1%) as coinfected, while RV15 detected 27 specimens (60.0%) as coinfected. AdvanSure assay demonstrated exquisite performance for the detection of RVs and will be a valuable tool for the management of RV infection.

    Original languageEnglish
    Pages (from-to)14-18
    Number of pages5
    JournalDiagnostic Microbiology and Infectious Disease
    Volume79
    Issue number1
    DOIs
    Publication statusPublished - 2014 May

    Bibliographical note

    Funding Information:
    Funding: This study was partly supported by a grant of the Transgovernmental Enterprise for Pandemic Influenza in Korea ‚ which was the part of Korea Healthcare technology R&D Project by Ministry of Health & Welfare‚ Republic of Korea (grant no.: A103001 ).

    Keywords

    • Multiplex real-time PCR
    • Respiratory virus
    • Viral isolation

    ASJC Scopus subject areas

    • Microbiology (medical)
    • Infectious Diseases

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