Evaluation of the overall process on bioethanol production from miscanthus hydrolysates obtained by dilute acid pretreatment

Hah Young Yoo, Xiaoguang Yang, Dong Sup Kim, Soo Kweon Lee, Pongtharin Lotrakul, Sehanat Prasongsuk, Hunsa Punnapayak, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


In this study, dilute sulfuric acid pretreatment was performed to improve the sugars recovery from Korean Miscanthus straw. The effect of pretreatment conditions on solubilized xylose was fundamentally investigated for the efficient removal of xylan. The optimal conditions were determined using a statistical method, and were shown to be a temperature of 121.6°C, an acid concentration of 1.1%, and a reaction time of 12.8 min. The combined severity factor was shown to be 1.1 under the optimum conditions. Following the pretreatment, the solubilized xylose in liquid fraction was found to be 71.2%, and about 72.6% of the solid was recovered. After enzymatic hydrolysis, about 86.4% glucose conversion was achieved when the pretreated biomass was used as a substrate, with the conversion being improved 4-fold compared with the control (untreated). The hydrolysates, approximately 10 g/L glucose, were applied to the fermentation of Saccharomyces cerevisiae K35, and the ethanol yield was about 96%. The overall process was evaluated based on the material balance, and the results show that approximately 172 g bioethanol can be produced when 1,000 g Miscanthus straw is loaded into the process.

Original languageEnglish
Pages (from-to)733-742
Number of pages10
JournalBiotechnology and Bioprocess Engineering
Issue number6
Publication statusPublished - 2016 Nov 1


  • bioethanol
  • dilute acid pretreatment
  • mass balance
  • miscanthus
  • process

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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