Evidence for the primo vascular system above the epicardia of rat hearts

Ho Sung Lee; Jeong Yim Lee; Dae In Kang; Se Hoon Kim; Inhyung Lee; Sang Hyun Park; Seung Zhoo Yoon; Yeon Hee Ryu; Byung Cheon Lee

doi:10.1155/2013/510461

Evidence for the primo vascular system above the epicardia of rat hearts

Ho Sung Lee, Jeong Yim Lee, Dae In Kang, Se Hoon Kim, Inhyung Lee, Sang Hyun Park, Seung Zhoo Yoon, Yeon Hee Ryu, Byung Cheon Lee

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We for the first time reported evidence for the existence of a novel network, a PVS, abovethe epicardium of the rat heart. (1) We were consecutively able to visualize the PVs and the PNs above the epicardial spaces of five rats' hearts by using Cr-Hx spraying or injection. (2) Hematoxylin and eosin (H&E) and toluidine blue staining of the PVs and the PNs showed that they consisted of a basophilic matrix; specifically the PNs contained several mast cells, some of which were degranulating into pericardial space. Also, 4′, 6-diamidino-2 phenylindole (DAPI) images of the PVs and the PNs showed that they contained various kinds of cells. (3) Transmission electron microscopic (TEM) longitudinal image of the PVs showed that the sinuses contained many granules with high-electron-density cores in parallel with putative endothelial cells. (4) TEM images of the PNs demonstrated that they consisted of lumen-containing cells surrounded by fibers and that they had mast cells that were degranulating toward the epicardium of the rat heart. The above data suggest that mast-cells-containing novel network exists above the epicardium of the rat heart.

Original language	English
Article number	510461
Journal	Evidence-based Complementary and Alternative Medicine
Volume	2013
DOIs	https://doi.org/10.1155/2013/510461
Publication status	Published - 2013
Externally published	Yes

ASJC Scopus subject areas

Complementary and alternative medicine

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title = "Evidence for the primo vascular system above the epicardia of rat hearts",

abstract = "We for the first time reported evidence for the existence of a novel network, a PVS, abovethe epicardium of the rat heart. (1) We were consecutively able to visualize the PVs and the PNs above the epicardial spaces of five rats' hearts by using Cr-Hx spraying or injection. (2) Hematoxylin and eosin (H&E) and toluidine blue staining of the PVs and the PNs showed that they consisted of a basophilic matrix; specifically the PNs contained several mast cells, some of which were degranulating into pericardial space. Also, 4′, 6-diamidino-2 phenylindole (DAPI) images of the PVs and the PNs showed that they contained various kinds of cells. (3) Transmission electron microscopic (TEM) longitudinal image of the PVs showed that the sinuses contained many granules with high-electron-density cores in parallel with putative endothelial cells. (4) TEM images of the PNs demonstrated that they consisted of lumen-containing cells surrounded by fibers and that they had mast cells that were degranulating toward the epicardium of the rat heart. The above data suggest that mast-cells-containing novel network exists above the epicardium of the rat heart.",

author = "Lee, {Ho Sung} and Lee, {Jeong Yim} and Kang, {Dae In} and Kim, {Se Hoon} and Inhyung Lee and Park, {Sang Hyun} and Yoon, {Seung Zhoo} and Ryu, {Yeon Hee} and Lee, {Byung Cheon}",

year = "2013",

doi = "10.1155/2013/510461",

language = "English",

volume = "2013",

journal = "Evidence-based Complementary and Alternative Medicine",

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AU - Lee, Ho Sung

AU - Lee, Jeong Yim

AU - Kang, Dae In

AU - Kim, Se Hoon

AU - Lee, Inhyung

AU - Park, Sang Hyun

AU - Yoon, Seung Zhoo

AU - Ryu, Yeon Hee

AU - Lee, Byung Cheon

PY - 2013

Y1 - 2013

N2 - We for the first time reported evidence for the existence of a novel network, a PVS, abovethe epicardium of the rat heart. (1) We were consecutively able to visualize the PVs and the PNs above the epicardial spaces of five rats' hearts by using Cr-Hx spraying or injection. (2) Hematoxylin and eosin (H&E) and toluidine blue staining of the PVs and the PNs showed that they consisted of a basophilic matrix; specifically the PNs contained several mast cells, some of which were degranulating into pericardial space. Also, 4′, 6-diamidino-2 phenylindole (DAPI) images of the PVs and the PNs showed that they contained various kinds of cells. (3) Transmission electron microscopic (TEM) longitudinal image of the PVs showed that the sinuses contained many granules with high-electron-density cores in parallel with putative endothelial cells. (4) TEM images of the PNs demonstrated that they consisted of lumen-containing cells surrounded by fibers and that they had mast cells that were degranulating toward the epicardium of the rat heart. The above data suggest that mast-cells-containing novel network exists above the epicardium of the rat heart.

AB - We for the first time reported evidence for the existence of a novel network, a PVS, abovethe epicardium of the rat heart. (1) We were consecutively able to visualize the PVs and the PNs above the epicardial spaces of five rats' hearts by using Cr-Hx spraying or injection. (2) Hematoxylin and eosin (H&E) and toluidine blue staining of the PVs and the PNs showed that they consisted of a basophilic matrix; specifically the PNs contained several mast cells, some of which were degranulating into pericardial space. Also, 4′, 6-diamidino-2 phenylindole (DAPI) images of the PVs and the PNs showed that they contained various kinds of cells. (3) Transmission electron microscopic (TEM) longitudinal image of the PVs showed that the sinuses contained many granules with high-electron-density cores in parallel with putative endothelial cells. (4) TEM images of the PNs demonstrated that they consisted of lumen-containing cells surrounded by fibers and that they had mast cells that were degranulating toward the epicardium of the rat heart. The above data suggest that mast-cells-containing novel network exists above the epicardium of the rat heart.

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Evidence for the primo vascular system above the epicardia of rat hearts

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