Evolution of CRISPR towards accurate and efficient mammal genome engineering

Seuk Min Ryu, Junseok W. Hur, Kyoungmi Kim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction.

Original languageEnglish
Pages (from-to)475-481
Number of pages7
JournalBMB reports
Volume52
Issue number8
DOIs
Publication statusPublished - 2019

Bibliographical note

Funding Information:
This study was supported by the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial fund, Korea university grant (K1804351), and the Bio & Medical Technology Development Program of the National Research Foundation (NRF) of Korea (NRF-2018M3A9H3021707, NRF-2018R1D1A1B07048434, and NRF-2014M3A9D5A01075128).

Publisher Copyright:
© 2019 by the The Korean Society for Biochemistry and Molecular Biology.

Keywords

  • CRISPR
  • DNA double-strand break
  • Genome editing
  • HDR
  • NHEJ

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Evolution of CRISPR towards accurate and efficient mammal genome engineering'. Together they form a unique fingerprint.

Cite this